Phospho-Gab1 (Tyr659) Antibody

• 产品编号：CST-12745S      品牌：CST       原厂货号：12745S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phosho-Gab1 (Tyr659) Antibody recognizes endogenous levels of Gab1 protein only when phosphorylated at Tyr659.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr659 of human Gab1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phosho-Gab1 (Tyr659) Antibody recognizes endogenous levels of Gab1 protein only when phosphorylated at Tyr659.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr659 of human Gab1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9)

1. Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
2. Weidner, K.M. et al. (1996) Nature 384, 173-176.
3. Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
4. Ingham, R.J. et al. (2001) J Biol Chem 276, 12257-65.
5. Lehr, S. et al. (1999) Biochemistry 38, 151-159.
6. Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923.
7. Yu, C.F. et al. (2001) J Biol Chem 276, 32552-8.
8. Gual, P. et al. (2000) Oncogene 19, 1509-18.
9. Cunnick, J.M. et al. (2001) J Biol Chem 276, 24380-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody