Human IL-17A Neutralizing (D13C2) Rabbit mAb

• 产品编号：CST-12739S      品牌：CST       原厂货号：12739S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Specificity / Sensitivity

Human IL-17A Neutralizing (D13C2) Rabbit mAb binds to human IL-17A (hIL-17A) and neutralizes its ability to induce IL-6 production in human dermal fibroblast cells. This antibody shows 50% cross-reactivity with human IL-17A/F. This antibody does not cross-react with mouse IL-17A, human IL-25 or mouse IL-25 .

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human IL-17A protein.

Description

Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. Human IL-17A Neutralizing (D13C2) Rabbit mAb has been shown to neutralize the production of IL-6 from human dermal fibroblast cells in vitro with an ND₅₀ in the range of 8-50 ng/ml.

原厂资料：

Specificity / Sensitivity

Human IL-17A Neutralizing (D13C2) Rabbit mAb binds to human IL-17A (hIL-17A) and neutralizes its ability to induce IL-6 production in human dermal fibroblast cells. This antibody shows 50% cross-reactivity with human IL-17A/F. This antibody does not cross-react with mouse IL-17A, human IL-25 or mouse IL-25 .

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human IL-17A protein.

Description

Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. Human IL-17A Neutralizing (D13C2) Rabbit mAb has been shown to neutralize the production of IL-6 from human dermal fibroblast cells in vitro with an ND₅₀ in the range of 8-50 ng/ml.

Endotoxin

Less than 0.1 EU/µg of antibody

Formulation

Lyophilized from a 0.2 µm filtered solution in 10 mM HEPES with trehalose.

Background

IL-17A is a cystine-linked homodimeric pro-inflammatory cytokine produced by Th₁₇ cells, a distinct CD4+ T cell lineage (1,2). IL-17A stimulates the production of the pro-inflammatory cytokines IL-1β, TNF-α, and IL-6. IL-17A also induces production of the neutrophil chemoattractants IL-8, CXCL1, and CXCL6 thereby bridging adaptive and innate immunity (1,2). IL-17A is intimately involved in mucosal immunity against bacterial infections (1,3) and has a putative role in some autoimmune disorders (1,4). IL-17A effects appear to be exerted primarily through binding to the IL-17RA (5). IL-17A binding induces production of cytokines, chemokines and other proteins through activation of the Erk1/2 MAP kinase, PI3K/Akt, p38, and NF-κB pathways (3,4,6).

注意事项：

Storage: Store lyophilized material at -20ºC. After reconstitu-
tion, recommended storage at 4ºC for 1 month or -20ºC for 6
months. Avoid repeated freeze/thawing