PathScan^? Phospho-Ron (panTyr)?Sandwich ELISA Kit

• 产品编号：CST-12737C      品牌：CST       原厂货号：12737C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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描述：

当人细胞中酪氨酸残基磷酸化时，PathScan® Phospho-Ron (panTyr) Sandwich ELISA Kit能够检测内源性水平的Ron蛋白，见图1.试剂盒的灵敏度见图2.本试剂盒用来检测指示物种的蛋白，即通过内部测试确定的那些，也可以用来检测来自其他物种的同源蛋白。PathScan® Phospho-Ron (panTyr) Sandwich ELISA Kit采用固相夹心酶联免疫吸附试验（ELISA），能够检测内源性水平的酪氨酸磷酸化Ron蛋白。微孔包被Ron兔单克隆抗体。与细胞裂解物孵育后，Ron蛋白（磷酸化和非磷酸化）由包被的抗体捕获。充分洗涤后，加入磷酸化酪氨酸小鼠检测单克隆抗体，以检测捕获得酪氨酸磷酸化Ron蛋白。然后，使用抗小鼠IgG，HRP-标记的抗体，识别结合的检测抗体。加入HRP底物TMB进行显色。显色的吸光度值与酪氨酸磷酸化Ron蛋白的量成正比。试剂盒中的抗体为试剂盒特异定制配方。Ron是受体酪氨酸激酶Met原癌基因家族的一员，该家族还包括Stk、 c-Met和 c-Sea。有功能的Ron是由一个40 kDa 的α链和一个150 kDa的β链组成的异二聚体。Ron最初在细胞中是作为单链合成的，然后pro-Ron的前体被切割成两条活性链。Α链完全位于胞外，而β链横跨细胞膜并含胞内酪氨酸激酶和调控元件 (1,2)。Ron介导和细胞运动、粘附、增殖以及凋亡相关的许多信号通路。Ron激活的下游信号通路包括ras/丝裂原活化蛋白激酶(MAPK)、磷脂酰肌醇-3激酶(PI3K)/Akt和粘着斑激酶(FAK)通路。Ron的激活也能显著增加c-Src的活性，c-Src是细胞周期进程、运动、成管和存活中的一个信号中介（3，4）。有研究表明，Ron在胚胎发育和肿瘤转移过程中有重要功能（5）。

1. Ronsin, C. et al. (1993) Oncogene 8, 1195-1202.
2. Gaudino, G. et al. (1994) EMBO J. 13, 3524-3532.
3. Wang, M.H. et al. (1996) Oncogene 13, 2167-2175.
4. Danilkovitch-Miagkova, A. (2003) Curr. Cancer Drug Targets 3, 31-40.
5. Leonard, E.J. (1997) Ciba Found. Symp. 212, 183-191; discussion 192-197.

原厂资料：

Specificity / Sensitivity

PathScan® Phospho-Ron (panTyr) Sandwich ELISA Kit detects endogenous levels of Ron protein when phosphorylated at Tyr residues in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

PathScan® Phospho-Ron (panTyr) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of tyrosine-phosphorylated Ron proteins. A Ron Rabbit mAb has been coated onto the microwells. After incubation with cell lysates, Ron protein (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, Phospho-Tyrosine Mouse Detection Antibody is added to detect captured tyrosine-phosphorylated Ron protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of tyrosine-phosphorylated Ron protein. 
 Antibodies in kit are custom formulations specific to kit.

Background

Ron is a member of the Met protooncogene family of receptor tyrosine kinases, which also includes Stk, c-Met, and c-Sea. The functional Ron is a heterodimer composed of a 40 kDa α chain and a 150 kDa β chain. Ron is initially synthesized in the cells as a single-chain, pro-Ron precursor that is cleaved into the two active chains. The α chain is completely extracellular, whereas the β chain traverses the cell membrane and contains the intracellular tyrosine kinase and regulatory elements (1,2). Ron mediates multiple signaling cascades that involve cell motility, adhesion, proliferation, and apoptosis. The signaling pathways activated downstream of Ron include the ras/mitogen-activated protein kinase (MAPK), phosphatidyl inositol-3 kinase (PI3K)/Akt, and focal adhesion kinase (FAK) pathways. Ron activation can also significantly increase c-Src activity, a signaling intermediate involved in cell cycle progression, motility, angiogenesis and survival (3,4). The function of Ron has been shown to be important for embryological development as well as implicated in the progression and metastasis of tumors (5).

1. Ronsin, C. et al. (1993) Oncogene 8, 1195-1202.
2. Gaudino, G. et al. (1994) EMBO J. 13, 3524-3532.
3. Wang, M.H. et al. (1996) Oncogene 13, 2167-2175.
4. Danilkovitch-Miagkova, A. (2003) Curr. Cancer Drug Targets 3, 31-40.
5. Leonard, E.J. (1997) Ciba Found. Symp. 212, 183-191; discussion 192-197.

注意事项：

* 12 8-well modules -Each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately)