Galectin-3/LGALS3 Antibody

• 产品编号：CST-12733S      品牌：CST       原厂货号：12733S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Specificity / Sensitivity

Galectin-3/LGALS3 antibody recognizes endogenous levels of total galectin-3/LGALS3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human galectin-3/LGALS3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

原厂资料：

Specificity / Sensitivity

Galectin-3/LGALS3 antibody recognizes endogenous levels of total galectin-3/LGALS3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human galectin-3/LGALS3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Galectins are a family of β-galactose binding proteins that are characterized by an affinity for poly-N-acetyllactosamine-enriched glycoconjugates and a carbohydrate-binding site (1,2). Members of the galectin family have been implicated in a variety of biological functions including cell adhesion (3), growth regulation (4), cytokine production (5), T-cell apoptosis (6), and immune responses (7).

Galectin-3/LGALS3 is involved in several diverse biological functions. Galectin-3/LGALS3 binds IgE (8). Galectin-3/LGALS3 is an unusual protein in that can be found both extracellularly and intracellularly. Intracellularly, galectin-3/LGALS3 can localize to the cytoplasm, nucleus, or both, depending on cell type and experimental conditions. Nuclear galectin-3/LGALS3 has been identified as a pre-mRNA splicing factor (9). Galectin-3/LGALS3 production has been shown to increase during inflammation and in obesity, and the protein itself can have an inflammatory effect under certain conditions (10). Galectin-3/LGALS3 forms a complex with α3, β1 integrin to act as a surface receptor on endothelial cells for the NG2 proteoglycan, which triggers cell motility and angiogenesis (11). In addition to these functions, galectin-3/LGALS3 is also a required factor for the terminal differentiation of epithelial cells (12).

1. Barondes, S.H. et al. (1994) Cell 76, 597-8.
2. Barondes, S.H. et al. (1994) J Biol Chem 269, 20807-10.
3. Offner, H. et al. (1990) J Neuroimmunol 28, 177-84.
4. Wells, V. and Mallucci, L. (1991) Cell 64, 91-7.
5. Filer, A. et al. (2009) Arthritis Rheum 60, 1604-14.
6. Perillo, N.L. et al. (1995) Nature 378, 736-9.
7. Cooper, D.N. et al. (1991) J Cell Biol 115, 1437-48.
8. Platzer, B. et al. (2011) Immunol Lett 141, 36-44.
9. Haudek, K.C. et al. (2010) Biochim Biophys Acta 1800, 181-9.
10. Pang, J. et al. (2013) PLoS One 8, e57915.
11. Fukushi, J. et al. (2004) Mol Biol Cell 15, 3580-90.
12. Hikita, C. et al. (2000) J Cell Biol 151, 1235-46.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody