Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb

• 产品编号：CST-12716S      品牌：CST       原厂货号：12716S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb 兔单抗可以识别Thr286被磷酸化的内源性的CamKII-α 总蛋白。抗体也可以识别Thr287被磷酸化的内源性CamKII-β 和CamKII-γ蛋白。该单克隆抗体由合成的人CamKII-β蛋白Thr287邻近磷酸肽段免疫动物而获得。CaMKII的是一种钙/钙调素活化蛋白激酶家族的重要成员，在神经突触的刺激和T细胞受体信号通路中起作用（1,2）。CaMKII具有催化和调节功能域。与Ca2+/钙调蛋白调节功能域的结合，可以产生自身抑制效果，并激活激酶（3）。活化的CaMKII进一步在Thr286自身磷酸化，呈现出其具有的激酶活性（3）。CaMKII的苏氨酸磷酸化状态，可通过PP1/PKA调节。PP1（蛋白磷酸酶1）可以对磷酸化的CaMKII在Thr286进行去磷酸化。PKA（蛋白激酶A）通过对PP1的抑制作用防止这种去磷酸化（4）。

1. Hughes, K. et al. (2001) J Biol Chem 276, 36008-13.
2. Barria, A. et al. (1997) Science 276, 2042-2045.
3. Barkai, U. et al. (2000) Mol. Endocrinol. 14, 554-563.
4. Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.

原厂资料：

Specificity / Sensitivity

Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb recognizes endogenous levels of CamKII-α protein only when phosphorylated at Thr286. This antibody also recognizes endogenous levels of CamKII-β and CamKII-γ protein only when phosphorylated at Thr287.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr287 of human CamKII-β protein.

Background

CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca²⁺/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4).

1. Hughes, K. et al. (2001) J Biol Chem 276, 36008-13.
2. Barria, A. et al. (1997) Science 276, 2042-2045.
3. Barkai, U. et al. (2000) Mol. Endocrinol. 14, 554-563.
4. Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.