Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb

• 产品编号：CST-12686S      品牌：CST       原厂货号：12686S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb可以识别Ser112被磷酸化的MCM3蛋白。抗体在处理间期和有丝分裂期细胞时能够检测到一条200 kDa的未知源性条带。Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb可以识别Ser112被磷酸化的MCM3蛋白。抗体在处理间期和有丝分裂期细胞时能够检测到一条200 kDa的未知源性条带。单克隆抗体由合成肽段免疫动物产生，该肽段与人MCM3的112位丝氨酸邻近残基序列一致。微染色体维持蛋白（MCM）2-7是一个由6个相关蛋白组成的家族，在DNA复制起始和延伸过程中是必须的。MCM2-7彼此结合形成异二聚体MCM复合物，被认为是DNA复制叉前进过程中的复制解旋酶(1-5).这个复合物是前复制复合物（pre-RC）的重要组成部分（1）。Cdc6和CDT1在有丝分裂/G1前期集合MCM复合物到起始识别复合物（ORC）形成pre-RC使DNA能够复制（2）。每个细胞周期中DNA只复制一次，因而帮助减少遗传变异和癌变细胞生长（3）。MCM2, MCM3, MCM4, 和MCM6亚基磷酸化可以调控MCM复合物活性起始DNA合成（6-8）。已经在体外证明，MCM3的112位丝氨酸被CDK1在有丝分裂后期/G1早期磷酸化会存进复合物形成染色质（8）。cdc7/dbf4磷酸化MCM2的139位丝氨酸能协助DNA复制（9）。DNA复制时MCM蛋白会被移除，通过抑制pre-RC再形成而保障染色质获得许可。研究表明MCM复合物可以保护复制叉的结构并在停滞后募集检验点蛋白协助重启复制（3,10）。

1. Lei, M. and Tye, B.K. (2001) J Cell Sci 114, 1447-54.
2. Lygerou, Z. and Nurse, P. (2000) Science 290, 2271-3.
3. Forsburg, S.L. (2004) Microbiol Mol Biol Rev 68, 109-31.
4. Tye, B.K. and Sawyer, S. (2000) J Biol Chem 275, 34833-6.
5. Labib, K. et al. (2000) Science 288, 1643-7.
6. Charych, D.H. et al. (2008) J Cell Biochem 104, 1075-86.
7. Masai, H. et al. (2006) J Biol Chem 281, 39249-61.
8. Lin, D.I. et al. (2008) Proc Natl Acad Sci USA 105, 8079-84.
9. Tsuji, T. et al. (2006) Mol Biol Cell 17, 4459-72.
10. Bailis, J.M. et al. (2008) Mol Cell Biol 28, 1724-38.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Hamster, Zebrafish, Dog

Specificity / Sensitivity

Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb recognizes endogenous levels of MCM3 protein only when phosphorylated at Ser112. This antibody also recognizes a 200 kDa band of unknown origin in both interphase and mitotic cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser112 of human MCM3 protein. 

Background

The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). CDK1 phosphorylation of MCM3 at Ser112 during late mitosis/early G1 phase has been shown to initiate complex formation and chromatin loading in vitro (8). Phosphorylation of MCM2 at serine 139 by cdc7/dbf4 coincides with the initiation of DNA replication (9). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,10).

1. Lei, M. and Tye, B.K. (2001) J Cell Sci 114, 1447-54.
2. Lygerou, Z. and Nurse, P. (2000) Science 290, 2271-3.
3. Forsburg, S.L. (2004) Microbiol Mol Biol Rev 68, 109-31.
4. Tye, B.K. and Sawyer, S. (2000) J Biol Chem 275, 34833-6.
5. Labib, K. et al. (2000) Science 288, 1643-7.
6. Charych, D.H. et al. (2008) J Cell Biochem 104, 1075-86.
7. Masai, H. et al. (2006) J Biol Chem 281, 39249-61.
8. Lin, D.I. et al. (2008) Proc Natl Acad Sci USA 105, 8079-84.
9. Tsuji, T. et al. (2006) Mol Biol Cell 17, 4459-72.
10. Bailis, J.M. et al. (2008) Mol Cell Biol 28, 1724-38.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.