MouseHis6Interleukin-21 (mHis6IL-21)

• 产品编号：CST-12670LC      品牌：CST       原厂货号：12670LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-2 Family 白细胞介素-2超家族
• 应用分类：

描述：

Source / Purification

Recombinant mouse _His6IL-21 (m_His6IL-21) Pro25-Ser146 (Accession #NP_068554.1) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant His6-tagged IL-21 has a calculated MW of 16,952. DTT-reduced and non-reduced protein migrate as 18-28 kDa polypeptides. Heterogeneity in SDS PAGE is due to glycosylation. The expected amino terminus of recombinant m_His6IL-21 was verified by amino acid sequencing.

Purity 

>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant m_His6IL-21. All lots are greater than 95% pure. 

Bioactivity

 The bioactivity of recombinant m_His6IL-21 was determined in a B9 cell proliferation assay. The ED₅₀ of each lot is between and 20-80 pg/ml.

原厂资料：

Source / Purification

Recombinant mouse _His6IL-21 (m_His6IL-21) Pro25-Ser146 (Accession #NP_068554.1) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant His6-tagged IL-21 has a calculated MW of 16,952. DTT-reduced and non-reduced protein migrate as 18-28 kDa polypeptides. Heterogeneity in SDS PAGE is due to glycosylation. The expected amino terminus of recombinant m_His6IL-21 was verified by amino acid sequencing.

Purity 

>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant m_His6IL-21. All lots are greater than 95% pure. 

Bioactivity

 The bioactivity of recombinant m_His6IL-21 was determined in a B9 cell proliferation assay. The ED₅₀ of each lot is between and 20-80 pg/ml.

Applications

Optimal concentration for the desired application should be determined by the user.

Endotoxin 

Less than 0.01 ng endotoxin/1 μg m_His6IL-21

Formulation

With carrier: Lyophilized from a 0.22 μm filtered 10 mM HEPES pH 7.0 solution containing 20 μg BSA per 1 μg m_His6IL-21. Carrier free: Lyophilized from a 0.22 μm filtered 10 mM HEPES pH 7.0 solution.

Background 

IL-21 is a pleiotropic type I cytokine that is produced by CD4+ T cells, Th17 cells and NKT cells (1,2). The IL-21 receptor is a heterodimer that consists of an IL-21R and the common γ chain, γc (1). IL-21/IL-21R signaling involves activation of JAKs1/3, STAT3 and, to a lesser extent, STAT5 and STAT1 (1,2). IL-21 promotes B cell proliferation, survival and differentiation into plasma cells (3,4). IL-21 activates the cytolytic functions of CD8+ T cells and NK cells, and has been shown in mouse models to play a key role in immunity to chronic viral infections and promote anti-tumor immunity (5-7).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.