CABIN1 (D2B9F) Rabbit mAb

• 产品编号：CST-12660S      品牌：CST       原厂货号：12660S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CABIN1 (D2B9F) Rabbit mAb兔单抗能够识别内源性CABIN1总蛋白水平。该单克隆抗体是采用合成的与人源CABIN1蛋白C末端氨基酸肽段免疫动物而产生的。Calcineurin结合蛋白CABIN1原本被认为是钙依赖性丝氨酸/苏氨酸磷酸酶Calcineurin的抑制剂。CABIN1能够在T细胞中抑制calcineurin信号通路、调节T细胞受体(TCR)信号通路、转录和凋亡(1-4)。CABIN1通过招募染色重塑酶来抑制心肌细胞增强因子2(MEF2)介导转录，另外其也能负调控肿瘤抑制基因P53的活性(6)。在基因毒性的应激反应中，CABIN1被降解，释放出对P53的抑制效应，使得P53发挥功能引起细胞的应激反应(7)。CABIN1作为高度保守的HIRA/UBN1/CABIN1/ASF1A（HUCA）组蛋白分子伴侣复合体的一部分，参与调节染色质的结构（8,9）。

1. Sun, L. et al. (1998) Immunity 8, 703-11.
2. Youn, H.D. and Liu, J.O. (2000) Immunity 13, 85-94.
3. Liu, W. et al. (2001) Eur J Immunol 31, 1757-64.
4. Kim, M.J. et al. (2002) Proc Natl Acad Sci U S A 99, 9870-5.
5. Jang, H. et al. (2007) J Biol Chem 282, 11172-9.
6. Jang, H. et al. (2009) Nat Struct Mol Biol 16, 910-5.
7. Choi, S.Y. et al. (2013) Nucleic Acids Res 41, 2180-2190.
8. Rai, T.S. et al. (2011) Mol Cell Biol 31, 4107-18.
9. Tang, Y. et al. (2012) Biochemistry 51, 2366-77.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

CABIN1 (D2B9F) Rabbit mAb recognizes endogenous levels of total CABIN1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CABIN1 protein.

Background

Calcineurin binding protein CABIN1 was originally identified as an inhibitor of the calcium-dependent serine/threonine phosphatase, calcineurin. CABIN1 inhibits calcineurin signaling in T cells, regulating T cell receptor (TCR) signaling, transcription, and apoptosis (1-4). CABIN1 represses myocyte enhancer factor 2 (MEF2)-mediated transcription by recruiting chromatin remodeling enzymes (5), and also negatively regulates the activity of the tumor suppressor p53 (6). In response to genotoxic stress, CABIN1 is degraded and releases its inhibition of p53, allowing p53 to elicit cellular stress responses (7). CABIN1 is also involved in regulation of chromatin structure as part of the highly conserved HIRA/UBN1/CABIN1/ASF1A (HUCA) histone chaperone complex (8,9).

1. Sun, L. et al. (1998) Immunity 8, 703-11.
2. Youn, H.D. and Liu, J.O. (2000) Immunity 13, 85-94.
3. Liu, W. et al. (2001) Eur J Immunol 31, 1757-64.
4. Kim, M.J. et al. (2002) Proc Natl Acad Sci U S A 99, 9870-5.
5. Jang, H. et al. (2007) J Biol Chem 282, 11172-9.
6. Jang, H. et al. (2009) Nat Struct Mol Biol 16, 910-5.
7. Choi, S.Y. et al. (2013) Nucleic Acids Res 41, 2180-2190.
8. Rai, T.S. et al. (2011) Mol Cell Biol 31, 4107-18.
9. Tang, Y. et al. (2012) Biochemistry 51, 2366-77.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to detect this antibody