IDH2 (D7H6Q) Rabbit mAb

• 产品编号：CST-12652S      品牌：CST       原厂货号：12652S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IDH2 (D7H6Q) Rabbit mAb兔单抗能够检测内源性IDH2总蛋白水平。该抗体也能够检测过表达的IDH1蛋白。该单克隆抗体是由合成的人源IDH2蛋白N末端肽段免疫动物而制备的。IDH2是三种异柠檬酸脱氢酶之一，催化异柠檬酸的氧化脱羧，产生二氧化碳和α-酮戊二酸（α-KG）。 IDH2和IDH1是 NADP+依赖家族的成员。 IDH2是一种粒线体酶，也作用于TCA循环（1,2）。IDH1和IDH2的体细胞突变是在胶质母细胞瘤中初步被确定（3）。更多研究发现，在急性髓细胞白血病和其他恶性肿瘤中，IDH1和IDH2的突变处于较低频率（4,5）。 IDH1和IDH2突变后获得了新的功能，在癌细胞中积累和分泌癌代谢物R-2-羟基戊二酸（2HG）（6,7）。

1. Wise, D.R. et al. (2011) Proc Natl Acad Sci U S A 108, 19611-6.
2. Filipp, F.V. et al. (2012) Pigment Cell Melanoma Res 25, 375-83.
3. Parsons, D.W. et al. (2008) Science 321, 1807-12.
4. Abbas, S. et al. (2010) Blood 116, 2122-6.
5. Paschka, P. et al. (2010) J Clin Oncol 28, 3636-43.
6. Watanabe, T. et al. (2009) Am J Pathol 174, 1149-53.
7. Pardanani, A. et al. (2010) Leukemia 24, 1370-2.

原厂资料：

Specificity / Sensitivity

IDH2 (D7H6Q) Rabbit mAb recognizes endogenous levels of total IDH2 protein. This antibody also recognizes overexpressed IDH1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human IDH2 protein.

Background

IDH2 is one of three isocitrate dehydrogenases that catalyze the oxidative decarboxylation of isocitrate to produce CO2 and α-ketoglutarate (α-KG). IDH2 and IDH1 are the NADP+-dependent family members. IDH2 is a mitochodrial enzyme that also functions in the TCA cycle (1,2). Somatic mutations of IDH1 and IDH2 were initially identified in glioblastoma (3). More research studies have identified IDH1 and IDH2 mutations in acute myeloid leukemia and other malignancies at lower frequency (4,5). Mutations of IDH1 and IDH2 confer a gain of function, enzymatic acitivity that results in the accumulation and secretion of the oncometabolite R-2-hydroxyglutarate (2HG) in cancer cells (6,7).

1. Wise, D.R. et al. (2011) Proc Natl Acad Sci U S A 108, 19611-6.
2. Filipp, F.V. et al. (2012) Pigment Cell Melanoma Res 25, 375-83.
3. Parsons, D.W. et al. (2008) Science 321, 1807-12.
4. Abbas, S. et al. (2010) Blood 116, 2122-6.
5. Paschka, P. et al. (2010) J Clin Oncol 28, 3636-43.
6. Watanabe, T. et al. (2009) Am J Pathol 174, 1149-53.
7. Pardanani, A. et al. (2010) Leukemia 24, 1370-2.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.