SignalSilence? PFKP siRNA I

• 产品编号：CST-12649S      品牌：CST       原厂货号：12649S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

G6PD (D5D2) Rabbit mAb兔单抗抑制人和猴的PFKP基因表达。CST公司的 SignalSilence ® PFKP siRNA I产品能够满足研究者通过RNA干扰方式特异性抑制PFKP基因表达的需求，该方法可以通过将双链RNA分子转移至细胞内而选择性沉默基因的表达。所有CST的SignalSilence ® siRNA产品都是经过严格的内部测试，并已通过western方法证明可以降低目标蛋白的表达。通过三苯分析监测寡核苷酸合成中的每一个碱基，以确保最佳的耦合效率。随后，该寡核苷酸经亲和固相萃取方式提纯。退火的RNA双链进一步通过质谱法进行分析，验证双链的确切组成。每个批次都会通过质谱分析与之前批次进行比对，最大程度确保批间一致性。磷酸果糖激酶(PFK)在糖酵解过程中催化6-磷酸果糖的磷酸化（1）。它有三种同工酶：肌肉型，肝脏型和血小板型（2,3）。血小板型的磷酸果糖激酶(PFKP)在多种细胞中表达（4,5）。研究发现，幼龄时发生PFKP基因变异的个体在成年后易患肥胖和糖尿病（6）。

1. Mediavilla, D. et al. (2008) J Biochem 144, 235-44.
2. Eto, K. et al. (1994) Biochem Biophys Res Commun 198, 990-8.
3. Hannemann, A. et al. (2005) Gene 345, 237-47.
4. Morrison, N. et al. (1992) Hum Genet 89, 105-6.
5. Vora, S. (1983) Isozymes Curr Top Biol Med Res 11, 3-23.
6. Morgan, A.R. et al. (2010) BMC Med Genet 11, 125.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

G6PD (D5D2) Rabbit mAb inhibits human and monkey PFKP expression.

G6PD (D5D2) Rabbit mAb兔单抗抑制人和猴的PFKP基因表达。

Description

SignalSilence® PFKP siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PFKP expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® PFKP siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. 
 Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Background

Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis (1). There are three isozymes: muscle-type, liver-type, and platelet-type (2,3). Platelet-type phosphofructokinase (PFKP) is expressed in various cell types (4,5). Research studies have shown that genetic variations in PFKP are associated with individuals born small for gestational age that are prone to obesity and diabetes later in adulthood (6).

1. Mediavilla, D. et al. (2008) J Biochem 144, 235-44.
2. Eto, K. et al. (1994) Biochem Biophys Res Commun 198, 990-8.
3. Hannemann, A. et al. (2005) Gene 345, 237-47.
4. Morrison, N. et al. (1992) Hum Genet 89, 105-6.
5. Vora, S. (1983) Isozymes Curr Top Biol Med Res 11, 3-23.
6. Morgan, A.R. et al. (2010) BMC Med Genet 11, 125.

注意事项：

Storage: PFKP siRNA I is supplied in RNAse-free water. Aliquot
and store at -20ºC.