Lefty1 (D7E3G) Rabbit mAb

• 产品编号：CST-12647S      品牌：CST       原厂货号：12647S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Lefty1 (D7E3G)Rabbit mAb兔单抗可以识别转染的总lefty1蛋白。此单克隆抗体由合成肽段免疫动物产生，该肽段与人lefty1蛋白的Pro108邻近氨基酸残基序列一致。Lefty蛋白是细胞信号分子TGF-β家族成员参与到了生长和发育（1,2）。Lefty的命名是由于它们在左右轴向决定以及特定表达在小鼠左侧胚胎。lefty1和lefty2含有cysteine-knot基序，这被认为是TGF-β相关蛋白的特点，但是缺乏形成配体二聚化的一个alpha-螺旋和半胱甘酸残基(3)。在脊椎动物发育早期，lefty通过抑制Smad2磷酸化以抑制TGF-β信号通路（4）。在分化早期lefty表达下调（5），lefty可以作为Nodal信号通路的胞外拮抗剂，Nodal是TGF-β的配体，对于左右模式发育和中胚层以及内胚层形成是至关重要的（6）。与TGF-β超家族的其他成员类似，lefty前蛋白会经过切割后释放一个具有生物活性的蛋白（7）。具有生物活性的42kDa lefty前体以及28 kDa多肽都能激活MAPK活性（7）。

1. Kingsley, D.M. (1994) Genes Dev 8, 133-46.
2. Heldin, C.H. et al. (1997) Nature 390, 465-71.
3. Meno, C. et al. (1997) Genes Cells 2, 513-24.
4. Ulloa, L. and Tabibzadeh, S. (2001) J Biol Chem 276, 21397-404.
5. Besser, D. (2004) J Biol Chem 279, 45076-84.
6. Schier, A.F. (2003) Annu Rev Cell Dev Biol 19, 589-621.
7. Ulloa, L. et al. (2001) J Biol Chem 276, 21387-96.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

Lefty1 (D7E3G) Rabbit mAb recognizes transfected levels of total lefty1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro108 of human lefty1 protein.

Background

Lefty proteins are members of the TGF-β family of cell signaling molecules that are involved in growth and development (1,2). Named for their role in left-right axis determination and their exclusive expression on the left side of the developing mouse embryo, lefty1 and lefty2 contain a cysteine-knot motif that is characteristic of TGF-β related proteins, but lack an alpha-helix and a cysteine residue critical for ligand dimerization (3). Early in vertebrate embryogenesis, lefty represses TGF-β signaling by inhibiting the phosphorylation of Smad2 following activation of the TGF-β receptor (4). Down-regulated very early upon differentiation (5), lefty proteins act as extracellular antagonists of the signaling pathway for Nodal, a TGF-β ligand critical for left-right patterning and formation of the mesoderm and endoderm (6). Similar to other members of the TGF-β superfamily, lefty proproteins undergo cleavage to release a bioactive protein (7). The biologically active 42 kDa lefty precursor and the 28 kDa polypeptide have been shown to induce MAPK activity (7).

1. Kingsley, D.M. (1994) Genes Dev 8, 133-46.
2. Heldin, C.H. et al. (1997) Nature 390, 465-71.
3. Meno, C. et al. (1997) Genes Cells 2, 513-24.
4. Ulloa, L. and Tabibzadeh, S. (2001) J Biol Chem 276, 21397-404.
5. Besser, D. (2004) J Biol Chem 279, 45076-84.
6. Schier, A.F. (2003) Annu Rev Cell Dev Biol 19, 589-621.
7. Ulloa, L. et al. (2001) J Biol Chem 276, 21387-96.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.