Btk (D3H5) Rabbit mAb (Biotinylated)

• 产品编号：CST-12624S      品牌：CST       原厂货号：12624S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

Specificity / Sensitivity

Btk (D3H5) Rabbit mAb (Biotinylated) recognizes endogenous levels of total Btk protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp195 of human Btk protein.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Btk (D3H5) Rabbit mAb #8547.

原厂资料：

Specificity / Sensitivity

Btk (D3H5) Rabbit mAb (Biotinylated) recognizes endogenous levels of total Btk protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp195 of human Btk protein.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Btk (D3H5) Rabbit mAb #8547.

Background

Bruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development (1,2). Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate (3-5). The membrane-localized Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinases, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation (6,7). The activation of Btk is negatively regulated by PKCβ through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation, and subsequent activation (8). The PKC inhibitory signal is likely to be a key determinant of the B cell receptor signaling threshold to maintain optimal Btk activity (8).

1. Khan, W.N. (2001) Immunol Res 23, 147-56.
2. Lewis, C.M. et al. (2001) Curr Opin Immunol 13, 317-25.
3. Salim, K. et al. (1996) EMBO J 15, 6241-50.
4. Rameh, L.E. et al. (1997) J Biol Chem 272, 22059-66.
5. Várnai, P. et al. (1999) J Biol Chem 274, 10983-9.
6. Rawlings, D.J. et al. (1996) Science 271, 822-5.
7. Park, H. et al. (1996) Immunity 4, 515-25.
8. Kang, S.W. et al. (2001) EMBO J 20, 5692-702.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.