MITF (D5G7V) Rabbit mAb

• 产品编号：CST-12590S      品牌：CST       原厂货号：12590S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MITF (D5G7V) Rabbit mAb兔单抗可以识别内源性的总MITF蛋白。预测该抗体可以识别所有的MITF亚型。此单克隆抗体由合成肽段免疫动物产生，该肽段与人MITF蛋白的Gly335邻近氨基酸残基序列一致。眼球相关转录因子(MITF)是一个碱性螺旋-环-螺旋锌指转录因子，最广为人知是它在黑色素细胞，眼科和破骨细胞发展中的作用(1-3)。人体中MITF可以在黑色素瘤中发挥作用(4)，MITF基因突变与2型Waardenburg 综合症，一种听觉-色素综合症表现为神经嵴源性干细胞发育缺陷(5)。MITF发现了至少12种亚型，表现出不同的细胞和组织特异性(6)。

1. Hershey, C.L. and Fisher, D.E. (2004) Bone 34, 689-96.
2. Nomura, S. et al. (2001) J Bone Miner Metab 19, 183-7.
3. Widlund, H.R. and Fisher, D.E. (2003) Oncogene 22, 3035-41.
4. Levy, C. et al. (2006) Trends Mol Med 12, 406-14.
5. Read, A.P. and Newton, V.E. (1997) J Med Genet 34, 656-65.
6. Tachibana, M. (2000) Pigment Cell Res 13, 230-40.

原厂资料：

Specificity / Sensitivity

MITF (D5G7V) Rabbit mAb recognizes endogenous levels of total MITF protein. The antibody is predicted to recognize all known isoforms.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly335 of human MITF protein.

Background

Microphthalmia-associated transcription factor (MITF) is a basic helix-loop-helix leucine zipper transcription factor that is most widely known for its roles in melanocyte, ophthalmic, and osteoclast development (1-3). In humans, MITF can function as a melanoma oncogene (4) and mutations in the corresponding MITF gene are associated with Waardenburg syndrome type 2, an auditory-pigmentary syndrome characterized by developmental defects in cells derived from neural crest (5). At least 12 isoforms of MITF have been identified, which exhibit differential patterns of expression of cell and tissue types (6).

1. Hershey, C.L. and Fisher, D.E. (2004) Bone 34, 689-96.
2. Nomura, S. et al. (2001) J Bone Miner Metab 19, 183-7.
3. Widlund, H.R. and Fisher, D.E. (2003) Oncogene 22, 3035-41.
4. Levy, C. et al. (2006) Trends Mol Med 12, 406-14.
5. Read, A.P. and Newton, V.E. (1997) J Med Genet 34, 656-65.
6. Tachibana, M. (2000) Pigment Cell Res 13, 230-40.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody