Adipogenesis Marker Antibody Sampler Kit

• 产品编号：CST-12589S      品牌：Cell Signaling Technology       原厂货号：12589S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

每个抗体都可以识别其各自内源性的靶蛋白。Adiponectin (C45B10) Rabbit mAb兔单抗可以识别内源性智联素总蛋白单体水平。它不会检测到大分子量的脂联素。Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗可以识别内源性所有亚型的acetyl-CoA carboxylase总蛋白水平。单抗由合成肽段免疫动物获得，抗体分别和human acetyl-CoA carboxylase α1的Ser523，人脂联素，人FABP4的序列，以及人脂肪酸合成酶的Gly46邻近序列，人脂周素蛋白Ile419邻近序列，人PPARγ的Asp69邻近序列一致。合成与人C/EBPα的序列一致的肽段免疫动物获得单克隆抗体。多抗使用蛋白A和肽段亲和层析纯化。Adipogenesis Marker Antibody Sampler试剂盒提供了一种经济的方式检测参与调控脂肪形成的蛋白。该试剂盒提供了足以进行4次western blot分析的一抗。脂肪细胞是脂肪组织的基础细胞组分，能够储存甘油三酯。脂肪形成是前脂肪细胞分化成脂肪细胞的过程。脂肪酸结合蛋白(FABPs) 可以作为细胞质脂类分子伴侣，通过结合脂肪酸和脂质运输到多个细胞通路(1,2). 脂联素是一种脂肪因子只表达在棕色和白色脂肪组织中，并分泌进入血液。它以三种主要形式存在：一个低分子量的三聚体，一个中等分子量的六聚体和一个高分子量的多聚体(3)。肥胖和胰岛素抵抗的小鼠和人体中脂联素表达会下降(4)，提示脂联素对于维持胰岛素敏感性是非常关键的。过氧化物酶体增殖物激活受体γ (PPARγ) 是一个转录激活因子选择性的表达在脂肪细胞，血管平滑肌细胞和巨噬细胞中(5,6)。乙酰-CoA羧化酶（ACC）是一个重要的脂肪酸合成和氧化酶，负责将乙酰-CoA羧化至丙二酸单酰辅酶A (7)。AMPK磷酸化Ser79或PKA磷酸化Ser1200能够抑制乙酰-CoA羧化酶的ACC酶活性(8)。ACC是一个可能的减肥药物靶点(9,10)。CCAAT/enhancer-binding proteins (C/EBPs)转录因子对于细胞分化，终末功能和炎症反应都非常关键(11)。GSK-3磷酸化C/EBPα的Thr222, Thr226, 和Ser230 是脂肪生成所必须的(12)。脂周素位于脂滴周围，保护脂滴不受脂肪酶威胁。已有证据显示PKA通过磷酸化脂周素调节脂解(13-17),引起构象改变使脂滴暴露给内源性的，激素敏感的脂肪酶(14)。因此，脂周素在脂肪储存中发挥了重要作用。脂肪酸合成酶(FASN)催化乙酰-CoA和丙二酰辅酶A 形成长链脂肪酸。FASN是一种活化的同二聚体具有七种不同的催化活性，在肝脏中产生脂肪以运输进入代谢活跃的组织或储存在脂肪组织中。在大多数别的人类组织中，FASN表达量很低，因为它们依赖于循环脂肪酸形成新的脂肪(18)。

原厂资料：

Specificity / Sensitivity

Each antibody recognizes endogenous total levels of its specific target protein. The Adiponectin (C45B10) Rabbit mAb detects endogenous levels of total adiponectin protein monomer. It will not detect higher molecular weight forms of adiponectin. The Acetyl-CoA Carboxylase (C83B10) Rabbit mAb detects endogenous levels of all isoforms of acetyl-CoA carboxylase protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase α1, to human adiponectin, to the sequence of human FABP4, to residues surrounding Gly46 of human fatty acid synthase, to residues surrounding Ile419 of human perilipin protein, or to residues surrounding Asp69 of human PPARγ. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human C/EBPα. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Adipogenesis Marker Antibody Sampler Kit provides an economical means to evaluate proteins involved in the regulation of adipogenesis. The kit includes enough antibody to perform four western blot experiments with each primary antibody.

Background

Adipocytes are the primary cellular component of adipose tissue and play a key role in the storage of triacylglycerol. Adipogenesis is the cellular process where preadipocytes differentiate into adipocytes.

Fatty acid binding proteins (FABPs) act as cytoplasmic lipid chaperones by binding fatty acids and lipids for transport to various cellular pathways (1,2). The predominant fatty acid binding protein found in adipocytes is FABP4.

Adiponectin is an adipokine expressed exclusively in brown and white adipocytes and is secreted into the blood. It exists in three major forms: a low molecular weight trimer, a medium molecular weight hexamer and a high molecular weight multimer (3). Decreased adiponectin levels are seen in obese and insulin-resistant mice and humans (4), suggesting that this adipokine is critical for maintenance of insulin sensitivity.

Peroxisome proliferator-activated receptor γ (PPARγ) is a transcriptional activator preferentially expressed in adipocytes, vascular smooth muscle cells, and macrophages (5,6).

Acetyl-CoA carboxylase (ACC) is a key fatty acid biosynthesis and oxidation enzyme that is responsible for the carboxylation of acetyl-CoA to malonyl-CoA, (7). Phosphorylation of acetyl-CoA carboxylase by AMPK at Ser79 or by PKA at Ser1200 inhibits ACC enzymatic activity (8). ACC is a potential target of anti-obesity drugs (9,10).

CCAAT/enhancer-binding proteins (C/EBPs) transcription factors are critical for cellular differentiation, terminal function, and the inflammatory response (11). Phosphorylation of C/EBPα at Thr222, Thr226, and Ser230 by GSK-3 may be required for adipogenesis (12).

Perilipin localizes to the periphery of lipid droplets and serves as a protective coating against lipases. Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin (13-17), resulting in a conformational change that exposes lipid droplets to endogenous, hormone-sensitive lipases (14). Hence, perilipin plays a pivotal role in lipid storage (14,17).

Fatty acid synthase (FASN) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA. FASN is active as a homodimer with seven different catalytic activities and produces lipids in the liver for export to metabolically active tissues or storage in adipose tissue. In most other human tissues, FASN is minimally expressed since they rely on circulating fatty acids for new structural lipid synthesis (18).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.