Lamin B1 (D4Q4Z) Rabbit mAb

• 产品编号：CST-12586S      品牌：CST       原厂货号：12586S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Lamin B1 (D4Q4Z) Rabbit mAb能够检测内源性水平lamin B1总蛋白。该单克隆抗体是由合成的人源lamin B1蛋白Leu118处肽段免疫动物而制备的。

核纤层蛋白Lamin是核膜的结构组分，对维持正常的细胞功能非常重要，例如细胞周期控制、DNA修复和染色质组装（1-3）。Lamins已细分为A和B型。A型核纤层蛋白由核纤层蛋白A和C组成，来源于选择性剪接。Lamin A/C能够被剪切成特异的大片段(41-50 kDa)和小片段 (28 kDa) ，这是细胞凋亡的标志（4,5）。B型核纤层蛋白由核纤层蛋白B1和B2组成，编码单独的基因（6-8）。在细胞凋亡时，核纤层蛋白B1也被半胱天冬酶酶切（9）。研究显示，核纤层蛋白B1基因的复制与神经功能紊乱成年期发病脑白质营养不良的发病机制相关（10）。

1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
2. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
3. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
4. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
5. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
6. Biamonti, G. et al. (1992) Mol Cell Biol 12, 3499-506.
7. Lin, F. and Worman, H.J. (1995) Genomics 27, 230-6.
8. Pollard, K.M. et al. (1990) Mol Cell Biol 10, 2164-75.
9. Chandler, J.M. et al. (1997) Biochem J 322 ( Pt 1), 19-23.
10. Padiath, Q.S. et al. (2006) Nat Genet 38, 1114-23.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Pig

Specificity / Sensitivity

Lamin B1 (D4Q4Z) Rabbit mAb recognizes endogenous levels of total lamin B1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu118 of human lamin B1 protein. 

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene LMNA. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (4,5). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8). Lamin B1 is also cleaved by caspases during apoptosis (9). Research studies have shown that duplication of the lamin B1 gene LMNB1 is correlated with pathogenesis of the neurological disorder adult-onset leukodystrophy (10).

1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
2. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
3. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
4. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
5. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
6. Biamonti, G. et al. (1992) Mol Cell Biol 12, 3499-506.
7. Lin, F. and Worman, H.J. (1995) Genomics 27, 230-6.
8. Pollard, K.M. et al. (1990) Mol Cell Biol 10, 2164-75.
9. Chandler, J.M. et al. (1997) Biochem J 322 ( Pt 1), 19-23.
10. Padiath, Q.S. et al. (2006) Nat Genet 38, 1114-23.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody