CXXC1 Antibody

• 产品编号：CST-12585S      品牌：CST       原厂货号：12585S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CXXC1 Antibody 能够检测内源性CXXC1的总蛋白水平。多克隆抗体是采用合成的与人源CXXC1 蛋白的Asp117残基周围序列相对应的肽段免疫动物而生产的。由蛋白质A和多肽亲和层析纯化而成。CXXC finger protein 1 (CXXC1, CGBP, CFP1) 是人SET1 组蛋白甲基转移酶复合体的一个关键业绩（1，2），该复合体能够甲基化组蛋白H3的Lys4位点从而产生一个转录激活启动子的标志（3,4）。CXXC1在CpG 岛含量丰富，在这里其可以选择性的与非甲基化 CpG motif 结合从而在global H3K4 甲基化与CpG岛建立连接（5）。研究揭示了CXXC1的一个功能，就是通过与DNMT1的直接相互作用从而维持胞嘧啶甲基化（6-9)。CXXC1表观遗传功能对于正常的胚胎发育是至关重要的。定点敲除小鼠的Cxxc1基因会导致早期胚胎致死性，而无Cxxc1基因的胚胎干细胞会呈现出更快的凋亡，并且在去除leukemia inhibitory factor LIF后无法在体外进行分化（6）。

1. Xu, C. et al. (2011) Nat Commun 2, 227.
2. Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
3. Miller, T. et al. (2001) Proc Natl Acad Sci U S A 98, 12902-7.
4. Clouaire, T. et al. (2012) Genes Dev 26, 1714-28.
5. Thomson, J.P. et al. (2010) Nature 464, 1082-6.
6. Carlone, D.L. et al. (2005) Mol Cell Biol 25, 4881-91.
7. Tate, C.M. et al. (2009) Mol Cell Biol 29, 3817-31.
8. Young, S.R. et al. (2006) J Biol Chem 281, 37034-44.
9. Butler, J.S. et al. (2008) DNA Cell Biol 27, 533-43.

原厂资料：

Specificity / Sensitivity

CXXC1 Antibody recognizes endogenous levels of total CXXC1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp117 of human CXXC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The CXXC finger protein 1 (CXXC1, CGBP, CFP1) is a key subunit of the human SET1 histone methyltransferase complex (1,2) that methylates histone H3 at Lys4 to create a mark of transcriptionally active promoters (3,4). CXXC1 is enriched at CpG islands where it selectively binds non-methylated CpG motifs to provide a link between global H3K4 methylation and CpG islands (5). Research studies have revealed a role for CXXC1 in the maintenance of cytosine methylation through direct interaction with DNMT1 (6-9). The epigenetic functions of CXXC1 are critical for normal embryonic development. Targeted deletion of the murine Cxxc1 gene results in early embryonic lethality while Cxxc1-null embryonic stem (ES) cells exhibit increased apoptosis and fail to undergo differentiation in vitro following withdrawal of leukemia inhibitory factor LIF (6).

1. Xu, C. et al. (2011) Nat Commun 2, 227.
2. Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
3. Miller, T. et al. (2001) Proc Natl Acad Sci U S A 98, 12902-7.
4. Clouaire, T. et al. (2012) Genes Dev 26, 1714-28.
5. Thomson, J.P. et al. (2010) Nature 464, 1082-6.
6. Carlone, D.L. et al. (2005) Mol Cell Biol 25, 4881-91.
7. Tate, C.M. et al. (2009) Mol Cell Biol 29, 3817-31.
8. Young, S.R. et al. (2006) J Biol Chem 281, 37034-44.
9. Butler, J.S. et al. (2008) DNA Cell Biol 27, 533-43.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody