Human His6IGFBP3 (hHis6IGFBP3)

• 产品编号：CST-12579LF      品牌：CST       原厂货号：12579LF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IGF 胰岛素样生长因子及其结合蛋白
• 应用分类：

描述：

在人293细胞中表达重组人_His6 IGFBP3 (h_His6IGFBP3) Gly28-Lys291 (Accession #NP_17936)。重组N端His标签的 hIGFBP3分子量30,7123 Da。DTT还原和非还原蛋白迁移大小为50 kDa。SDS-PAGE中低迁移率和均质性是由糖基化引起的。预测的重组h_His6IGFBP3的氨基端序列通过氨基酸测序确认。通过SDS-PAGE确认6 μg还原（+）和未还原（-）重组h_HisIGFBP3纯度大于>95%。所有的批次纯度都大于>95%。h_His6IGFBP3的活性通过抑制人皮肤成纤维细胞中IGF- I诱导的AKT磷酸化而测定。1 μg h_His6IGFBP3中内毒素低于0.01 ng。带载体：储存在含有 20 μg BSA 的20 mM Tris, pH 7.2 溶液中的1 μg hH_His6IGFBP3经过0.22 μm过滤后冻干。无载体：h_His6IGFBP3 i在 20 mM Tris, pH 7.2经过0.22 μm过滤后冻干。IGFBP3是一种多功能的蛋白，在调控IGFI/II活性，细胞增殖和细胞死亡上发挥关键作用。作为六个高亲和IGF结合蛋白之一，IGFBP3主要位于循环系统中，并和ALS结合成为复合物后与99%肝脏中的IGF- I结合 (1)。IGFBP3的蛋白降解过程增加了IGF I/II的生物活性(1)。但是，IGFBP3的某些生物性能是不依赖于IGF/IGF-IR信号通路的。体外实验表明IGFBP3通过增强ERK的磷酸化和sphingosine激酶介导的EGFR转化，从而增加可能引起了EGF诱发的乳腺癌(2,3)。相反的是，IGFBP3也被证明可以诱导凋亡并抑制NF-κB活性(4)。

原厂资料：

Source / Purification

Recombinant Human _His6IGFBP3 (h_His6IGFBP3) Gly28-Lys291 (Accession #NP_17936) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant N-terminally His6-tagged hIGFBP3 has a calculated MW of 30,7123 Da. DTT reduced and nonreduced protein migrate as 50 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino terminus of recombinant h_His6IGFBP3 was verified by amino acid sequencing.

Purity

>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant h_HisIGFBP3. All lots are greater than 95% pure.

Bioactivity

The bioactivity of h_His6IGFBP3 was determined by inhibition of IGF-I induced AKT phosphorylation in human dermal fibroblasts. The ED₅₀ of each lot is between 2.5-9 ng/ml.

Applications

Optimal concentration for the desired application should be determined by the user.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of h_His6IGFBP3 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg h_His6IGFBP3. Carrier free: Lyophilized from a 0.22 μm filtered solution of h_His6IGFBP3in 20 mM Tris, pH 7.2.

Background

IGFBP3 is a multifunctional protein that plays a key role in regulation of IGFI/II activity, cell proliferation, and death. One of six high-affinity IGF binding proteins, IGFBP3 is the major species in circulation and is bound in a complex with ALS to 99% of hepatic IGF-I (1). Proteolytic degradation of IGFBP3 increases the bioavailability and activity of the IGF I/II (1). However, some biological activities of IGFBP3 are independent of the IGF/IGF-IR axis. IGFBP3 potentiates EGF-induced breast cancer cell proliferation in vitro by enhancing ERK phosphorylation and sphingosine kinase-mediated EGFR trans-activation (2,3). Conversely, IGFBP3 has been shown to induce apoptosis and inhibit NF-κB activity (4).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid repeated freeze-thaw cycles.