USP13 (D4P3M) Rabbit mAb

• 产品编号：CST-12577S      品牌：CST       原厂货号：12577S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

USP13 (D4P3M) Rabbit mAb兔单抗识别内源性的USP13蛋白。该抗体与USP5/ISOT无交叉反应性。该单克隆抗体经合成人USP13蛋白的氨基末端邻近氨基酸残基序列一致的肽段，免疫动物产生。泛素化作用酶（UBEs）催化蛋白的泛素化，与去泛素化作用酶(DUB)的过程可逆(1,2)。5种DUB亚家族已经被确定，包括USP, UCH, OTU, MJD和JAMM酶。泛素羧基末端水解酶13(USP13;异构肽T-3)包含4种泛素-相关/翻译延伸因子EF1B，氨基末端(UBA)结构域和一种泛素-特异的加工蛋白酶(UBP)结构域。USP13的UBP结构域包含了一个催化位点，一个锌指结构域和2个UBA结构域。与其他USP家族成员类似，USP13包含了似乎在其催化机制中起作用的半胱氨酸和组氨酸。研究表明USP13通过对靶蛋白诸如BECN1和USP10的去泛素化作用，在自噬过程中起重要作用(3)。研究也表明USP13因其对MITF去泛素状态的调节能力，可能在黑色素瘤发病机理中起作用(4)。

1. Chen, M. et al. (2011) Proc Natl Acad Sci U S A 108, 9119-24.
2. Nijman, S.M. et al. (2005) Cell 123, 773-86.
3. Liu, J. et al. (2011) Cell 147, 223-34.
4. Zhao, X. et al. (2011) Nat Commun 2, 414.

原厂资料：

Specificity / Sensitivity

USP13 (D4P3M) Rabbit mAb recognizes endogenous levels of total USP13 protein. This antibody does not cross-react with USP5/ISOT.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human USP13 protein.

Background

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. Ubiquitin carboxyl-terminal hydrolase 13 (USP13; isopeptidase T-3) contains four ubiquitin-associated/translation elongation factor EF1B, amino terminal (UBA) domains and one ubiquitin-specific processing protease (UBP) domain. The UBP domain of USP13 contains a catalytic site, a zinc finger domain, and two UBA domains. Similar to other USP family members, USP13 contains cysteines and histidines that are likely involved in its catalytic mechanism. Studies show that USP13 plays a critical role in autophagy through the deubiquitination of target proteins such as BECN1 and USP10 (3). Research also suggests that USP13 is implicated in the pathogenesis of melanoma through its ability to regulate the ubiquitination status of MITF (4).

1. Chen, M. et al. (2011) Proc Natl Acad Sci U S A 108, 9119-24.
2. Nijman, S.M. et al. (2005) Cell 123, 773-86.
3. Liu, J. et al. (2011) Cell 147, 223-34.
4. Zhao, X. et al. (2011) Nat Commun 2, 414.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.