Cleaved Histone H3 (Thr22) (D7J2K) Rabbit mAb

• 产品编号：CST-12576S      品牌：CST       原厂货号：12576S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cleaved Histone H3 (Thr22) (D7J2K) Rabbit mAb兔单抗能够检测内源性的在Thr22位点剪切的组蛋白 H3水平。该抗体对Thr22位点剪切的组蛋白H3有强烈的偏好性，但是它也能与全长组蛋白H3产生微弱的交叉反应。该单克隆抗体是采用合成的与人源histone H3蛋白的Thr22残基周围序列相对应的肽段免疫动物而生产的。染色质结构的调节在多种DNA的直接激活如转录、DNA复制和修复的控制中有着重要作用 (1)。核染色质的基本单位核小体是由DNA分子盘绕在4种组蛋白H2A、H2B、H3和H4， 每一种组蛋白各二个分子(2,3)。在生理和环境应激下组蛋白的氨基端尾部经历不同的翻译后修饰例如乙酰化、甲基化、磷酸化和泛素化。这些修饰调节染色质更易接近效应蛋白，并且对于特定组蛋白修饰的结合位点可识别调节基因表达的效应蛋白(1,4,5)。在染色质修饰中这种改变和基因表达相关的体系结构的变化已经在胚胎干细胞分化被观察(6)。染色质修饰的其中一种方法可能在干细胞中被改变，这通过Cathepsin L涉及调节histone H3的水解。在分化的干细胞中Cathepsin L主要裂解histone H3氨基端尾部Thr22位点，这导致组蛋白修饰标记物的去除，然后很可能影响发育调节基因的表达模式(7)。

1. Smith, E. and Shilatifard, A. (2010) Mol Cell 40, 689-701.
2. Kornberg, R.D. (1974) Science 184, 868-71.
3. Kornberg, R.D. and Lorch, Y. (1999) Cell 98, 285-94.
4. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
5. Gardner, K.E. et al. (2011) J Mol Biol 409, 36-46.
6. Young, R.A. (2011) Cell 144, 940-54.
7. Duncan, E.M. et al. (2008) Cell 135, 284-94.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Xenopus, Bovine, Dog

Specificity / Sensitivity

Cleaved Histone H3 (Thr22) (D7J2K) Rabbit mAb recognizes endogenous levels of histone H3 protein when cleaved at Thr22. This antibody shows a strong preference for histone H3 protein when cleaved at Thr22, but also weakly recognizes full length histone H3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr22 of human histone H3 protein.

Background

Modulation of chromatin structure has a critical role in the control of various DNA directed activities such as transcription, DNA replication, and repair (1). The basic unit of chromatin, the nucleosome, consists of two turns of DNA wrapped around two copies each of four core histone proteins (H2A, H2B, H3, and H4) (2,3). Amino-terminal tails of histones undergo various post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination in response to physiological and environmental stimuli. These modifications modulate the accessibility of chromatin to effector proteins as well as act as binding sites for specific histone modification recognizing effector proteins that regulate gene expression (1,4,5). Such alterations in chromatin modifications and architecture that accompany gene expression changes have been observed during embryonic stem cell differentiation (6). One of the ways in which chromatin modifications may be altered in stem cells involves regulated proteolysis of histone H3 by Cathepsin L. Cathepsin L cleaves the histone H3 amino-terminal tail predominantly at Thr22 in differentiating stem cells, leading to removal of histone modification marks which could then influence the expression patterns of developmentally regulated genes (7).

1. Smith, E. and Shilatifard, A. (2010) Mol Cell 40, 689-701.
2. Kornberg, R.D. (1974) Science 184, 868-71.
3. Kornberg, R.D. and Lorch, Y. (1999) Cell 98, 285-94.
4. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
5. Gardner, K.E. et al. (2011) J Mol Biol 409, 36-46.
6. Young, R.A. (2011) Cell 144, 940-54.
7. Duncan, E.M. et al. (2008) Cell 135, 284-94.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.