Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb

• 产品编号：CST-12569S      品牌：CST       原厂货号：12569S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb可以识别83位丝氨酸被磷酸化的内源性vimentin蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人vimentin的83位丝氨酸邻近残基序列一致。细胞骨架由三种细胞纤维构成：微丝（肌动蛋白丝），中间丝和微管。中间丝的主要类型由它们的细胞特异性表达来区分：细胞角蛋白（上皮细胞），胶质原纤维酸性蛋白（GFAP）（神经胶质细胞），结蛋白（desmin）（骨骼，内脏，某些血管平滑肌细胞），波形蛋白（间充质起源），神经丝蛋白（神经元）。星形胶质细胞中GFAP和vimentin形成中间丝以调节它们的运动和形状（1）。尤其是vimentin中间丝出现在发育早期，GFAP中间丝是终末分化的成熟脑星形胶质细胞的标记（2）。研究表明vimentin在肉瘤中表达，而在肿瘤中不表达，它的表达与其他标记蛋白相结合用于研究区分这二者（3）。Vimentin的多变的结构和空间重组特性是对细胞外刺激的应激，帮助细胞协同调节多种信号通路（4）。肌肉细胞中Vimentin的56位丝氨酸磷酸化会调节vimentin中间丝的结构以应对肉瘤（5,6）。Vimentin和其他中间丝的重构对于淋巴细胞黏附及通过内皮细胞的迁移是非常重要的（7）。有丝分裂时，CDK1磷酸化vimentin的56位丝氨酸，该磷酸化提供了一个vimentin-PLK的结合位置。PLK随后磷酸化vimentin的83位丝氨酸，这可能作为一个记忆磷酸化位点在vimentin的解聚过程中发挥了重要的调控作用（8,9）。

1. Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
2. Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93.
3. Leader, M. et al. (1987) Histopathology 11, 63-72.
4. Helfand, B.T. et al. (2004) J Cell Sci 117, 133-41.
5. Tang, D.D. et al. (2005) Biochem J 388, 773-83.
6. Fomina, I.G. et al. (1990) Klin Med (Mosk) 68, 125-7.
7. Nieminen, M. et al. (2006) Nat Cell Biol 8, 156-62.
8. Yamaguchi, T. et al. (2005) J Cell Biol 171, 431-6.
9. Oguri, T. et al. (2006) Genes Cells 11, 531-40.
10. Zhu, Q.S. et al. (2011) Oncogene 30, 457-70.
11. Xue, G. and Hemmings, B.A. (2013) J Natl Cancer Inst 105, 393-404.
12. Yamaguchi, T. et al. (2005) J Cell Biol 171, 431-6.
13. Oguri, T. et al. (2006) Genes Cells 11, 531-40.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb recognizes endogenous levels of Vimentin protein only when phosphorylated at Ser83.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser83 of human vimentin protein.

Background

The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are distinguished by their cell-specific expression: cytokeratins (epithelial cells), glial fibrillary acidic protein (GFAP) (glial cells), desmin (skeletal, visceral, and certain vascular smooth muscle cells), vimentin (mesenchyme origin), and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). Research studies have shown that vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two (3). Vimentin's dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways (4). Phosphorylation of vimentin at Ser56 in smooth muscle cells regulates the structural arrangement of vimentin filaments in response to serotonin (5,6). Remodeling of vimentin and other intermediate filaments is important during lymphocyte adhesion and migration through the endothelium (7).

1. Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
2. Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93.
3. Leader, M. et al. (1987) Histopathology 11, 63-72.
4. Helfand, B.T. et al. (2004) J Cell Sci 117, 133-41.
5. Tang, D.D. et al. (2005) Biochem J 388, 773-83.
6. Fomina, I.G. et al. (1990) Klin Med (Mosk) 68, 125-7.
7. Nieminen, M. et al. (2006) Nat Cell Biol 8, 156-62.
8. Yamaguchi, T. et al. (2005) J Cell Biol 171, 431-6.
9. Oguri, T. et al. (2006) Genes Cells 11, 531-40.
10. Zhu, Q.S. et al. (2011) Oncogene 30, 457-70.
11. Xue, G. and Hemmings, B.A. (2013) J Natl Cancer Inst 105, 393-404.
12. Yamaguchi, T. et al. (2005) J Cell Biol 171, 431-6.
13. Oguri, T. et al. (2006) Genes Cells 11, 531-40.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.