Human His6IGFBP2

• 产品编号：CST-12568LF      品牌：CST       原厂货号：12568LF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 其它纯化的蛋白及酶
• 应用分类：

描述：

Source / Purification

 Recombinant Human _His6IGFBP2 (h_His6IGFBP2) Glu40-Gln328 (Accession #NP_18065) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

 Recombinant amino-terminally His6-tagged hIGFBP2 has a calculated MW of 32,998 Da. DTT-reduced and nonreduced protein migrates as a 37 kDa polypeptide. The expected amino terminus of recombinant h_His6IGFBP2 was verified by amino acid sequencing.

Purity

 >95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant h_HisIGFBP2. All lots are greater than 95% pure.

Bioactivity

 The bioactivity of h_His6IGFBP2 was determined by inhibition of IGF-I induced AKT phosphorylation in human dermal fibroblasts. The ED₅₀ of each lot is between 0.1 and 0.5 µg/ml.

原厂资料：

Source / Purification

 Recombinant Human _His6IGFBP2 (h_His6IGFBP2) Glu40-Gln328 (Accession #NP_18065) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

 Recombinant amino-terminally His6-tagged hIGFBP2 has a calculated MW of 32,998 Da. DTT-reduced and nonreduced protein migrates as a 37 kDa polypeptide. The expected amino terminus of recombinant h_His6IGFBP2 was verified by amino acid sequencing.

Purity

 >95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant h_HisIGFBP2. All lots are greater than 95% pure.

Bioactivity

 The bioactivity of h_His6IGFBP2 was determined by inhibition of IGF-I induced AKT phosphorylation in human dermal fibroblasts. The ED₅₀ of each lot is between 0.1 and 0.5 µg/ml.

Applications

 Optimal concentration for the desired application should be determined by the user.

 Endotoxin

Less than 0.01 ng endotoxin/1 μg h_His6IGFBP2.

Formulation

 With carrier: Lyophilized from a 0.22 μm filtered solution of h_His6IGFBP2 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg h_His6IGFBP2. Carrier free: Lyophilized from a 0.22 μm filtered solution of h_His6IGFBP2 in 20 mM Tris, pH 7.2.

Background

 IGFBP2 is a multifunctional protein that plays a key role in the regulation of IGFI/II activity, cell proliferation, and cell adhesion (1,2). One of six high-affinity IGF binding proteins, IGFBP2 is the second most abundant IGFBP species in circulation (1). IGFBP2 can antagonize IGF signaling, or directly stimulate cell proliferation depending on context and cell type (1,2). Many of these effects are IGF independent (2). Elevated serum levels of IGFBP2 have been reported in a variety of cancers (1,2). IGFBP2 has been implicated in angiogenesis in a murine melanoma model (3).

注意事项：

Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer