Rab7 (D95F2) XP??Rabbit mAb?(HRP Conjugate)

• 产品编号：CST-12561S      品牌：CST       原厂货号：12561S
• 产品分类：抗体 > 一抗 > 酶标记一抗 > 辣根过氧化物酶（HRP）
• 应用分类：

描述：

Specificity / Sensitivity

Rab7 (D95F2) XP^® Rabbit mAb (HRP Conjugate) detects endogenous levels of total Rab7 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu188 of human Rab7 protein.

Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Rab7 (D95F2) XP^® Rabbit mAb #9367.

原厂资料：

Specificity / Sensitivity

Rab7 (D95F2) XP^® Rabbit mAb (HRP Conjugate) detects endogenous levels of total Rab7 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu188 of human Rab7 protein.

Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Rab7 (D95F2) XP^® Rabbit mAb #9367.

Background

Rab7 and Rab9 are members of the Ras superfamily of small Rab GTPases (1). Both proteins are located in late endosomes, but exert different functions. Rab7 associates with the RIPL effector protein to control membrane trafficking from early to late endosome and to lysosomes (2,3). Rab7 also helps to regulate growth receptor endocytic trafficking and degradation (3,4), and maturation of phagosome and autophagic vacuoles (4-6). Rab9 interacts with its effector proteins p40 and TIP47 (7,8) to promote the MPR (mannose 6-phosphate receptor)-associated lysosomal enzyme transport between late endosomes and the trans Golgi network (9,10).

1. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
2. Feng, Y. et al. (1995) J Cell Biol 131, 1435-52.
3. Méresse, S. et al. (1995) J Cell Sci 108 ( Pt 11), 3349-58.
4. Ceresa, B.P. and Bahr, S.J. (2006) J Biol Chem 281, 1099-106.
5. Jäger, S. et al. (2004) J Cell Sci 117, 4837-48.
6. Méresse, S. et al. (1999) EMBO J 18, 4394-403.
7. Díaz, E. et al. (1997) J Cell Biol 138, 283-90.
8. Barbero, P. et al. (2002) J Cell Biol 156, 511-8.
9. Lombardi, D. et al. (1993) EMBO J 12, 677-82.
10. Riederer, M.A. et al. (1994) J Cell Biol 125, 573-82.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies