MRP2/ABCC2 (D9F9E) Rabbit mAb

• 产品编号：CST-12559S      品牌：CST       原厂货号：12559S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MRP2/ABCC2 (D9F9E) Rabbit mAb能够检测内源性的MRP2总蛋白水平。该单克隆抗体是由合成的人源MRP2蛋白精氨酸(864位)的肽段免疫动物而制备的。多药耐药相关蛋白2（MRP2），也称为CMRP，cMOAT和ABCC2，是一种ATP结合盒（ABC）转运蛋白，多药耐药（MRP）蛋白家族的一部分（1,2）。MRP蛋白是膜蛋白，作为细胞的有机阴离子泵参与着胞内癌症药物的去除（2）。MRP2对一些治疗癌症的药物有耐药性，如顺铂，依托泊苷，阿霉素，氨甲喋呤（3-5）。MRP2主要表达在肝脏顶膜（6-9）和肾脏近端小管（10）部位。它能够将肝细胞中的胆红素葡萄糖醛酸苷和有机阴离子分泌到胆汁中，这个过程是ATP-依赖的，是机体一种重要的排除内源和外源物质的方式。MRP2的功能缺失将导致一种名为杜宾-约翰逊综合症的常染色体隐性遗传疾病，其特征是肝细胞中阴离子共轭物的分泌缺陷以及黑色素像颜料一样的存在（11-13）。

1. Keppler, D. and Konig, J. (1997) FASEB J 11, 509-16.
2. Borst, P. et al. (2000) J Natl Cancer Inst 92, 1295-302.
3. Taniguchi, K. et al. (1996) Cancer Res 56, 4124-9.
4. Hooijberg, J.H. et al. (1999) Cancer Res 59, 2532-5.
5. Cui, Y. et al. (1999) Mol Pharmacol 55, 929-37.
6. Büchler, M. et al. (1996) J Biol Chem 271, 15091-8.
7. Paulusma, C.C. et al. (1996) Science 271, 1126-8.
8. Mayer, R. et al. (1995) J Cell Biol 131, 137-50.
9. Ito, K. et al. (1998) J Biol Chem 273, 1684-8.
10. Schaub, T.P. et al. (1997) J Am Soc Nephrol 8, 1213-21.
11. Dubin, I.N. and Johnson, F.B. (1954) Medicine (Baltimore) 33, 155-97.
12. Kartenbeck, J. et al. (1996) Hepatology 23, 1061-6.
13. Paulusma, C.C. et al. (1997) Hepatology 25, 1539-42.

原厂资料：

Specificity / Sensitivity

MRP2/ABCC2 (D9F9E) Rabbit mAb recognizes endogenous levels of total MRP2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg864 of human MRP2 protein.

Background

Multi-drug resistance protein 2 (MRP2), also known as cMRP, cMOAT, and ABCC2, is an ATP binding cassette (ABC) transporter and part of the multi-drug resistance (MRP) family (1,2). The MRP proteins are membrane proteins that function as organic anion pumps involved in the cellular removal of cancer drugs (2). MRP2 is associated with resistance to a number of cancer drugs, such as cisplatin, etoposide, doxorubicin, and methotrexate (3-5). MRP2 is predominately expressed on the apical membranes in the liver (6-9) and kidney proximal tubules (10). It is responsible for the ATP-dependent secretion of bilirubin glucuronides and other organic anions from hepatocytes into the bile, a process important for the excretion of endogenous and xenobiotic substances. Loss of MRP2 activity is the cause of Dubin-Johnson syndrome, an autosomal recessive disorder characterized by defects in the secretion of anionic conjugates and the presence of melanin like pigments in hepatocytes (11-13).

1. Keppler, D. and Konig, J. (1997) FASEB J 11, 509-16.
2. Borst, P. et al. (2000) J Natl Cancer Inst 92, 1295-302.
3. Taniguchi, K. et al. (1996) Cancer Res 56, 4124-9.
4. Hooijberg, J.H. et al. (1999) Cancer Res 59, 2532-5.
5. Cui, Y. et al. (1999) Mol Pharmacol 55, 929-37.
6. Büchler, M. et al. (1996) J Biol Chem 271, 15091-8.
7. Paulusma, C.C. et al. (1996) Science 271, 1126-8.
8. Mayer, R. et al. (1995) J Cell Biol 131, 137-50.
9. Ito, K. et al. (1998) J Biol Chem 273, 1684-8.
10. Schaub, T.P. et al. (1997) J Am Soc Nephrol 8, 1213-21.
11. Dubin, I.N. and Johnson, F.B. (1954) Medicine (Baltimore) 33, 155-97.
12. Kartenbeck, J. et al. (1996) Hepatology 23, 1061-6.
13. Paulusma, C.C. et al. (1997) Hepatology 25, 1539-42.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.