Tris-Glycine Transfer Buffer (10X)

• 产品编号：CST-12539S      品牌：CST       原厂货号：12539S
• 产品分类：生化试剂 > 生物缓冲液 > 蛋白生化分析缓冲液 > 印迹缓冲液
• 应用分类：

描述：

ris-Glycine Transfer Buffer (10X)在western blotting用于转膜液。产品运输和储存在室温下。1 x配方:25 mM Tris,192 mM甘氨酸,20%(v / v)甲醇,pH ~ 8.3。注意:甲醇不提供,但是必需的。

储存：室温储存10 X溶液，稳定24个月。稀释成1X并加入甲醇后保存于4°C1周。配制1L1 x transfer buffer:混合100 X transfer buffer,200毫升甲醇,800毫升双蒸水，保存于4ºC长达一个星期。本产品供应足够的材料配制成10升1 x的溶液。一般来说,20%甲醇是推荐的,但在转分子量大但密度低的蛋白时，减少甲醇浓度为5 - 10%。CST建议0.2μm孔隙大小尼龙膜在70伏特下转膜2小时。ris-Glycine Transfer Buffer (10X)是一种常用的免疫印迹缓冲液，用于将蛋白质从SDS-PAGE凝胶转移到尼龙膜或PVDF膜。合成基于被广泛接受的owbin transfer buffer (1)并用于CST推荐的湿转系统。

原厂资料：

Description

Tris-Glycine Transfer Buffer (10X) is used as a transfer buffer during western blotting. Product is shipped and stored at room temperature.

1X Formulation: 25 mM Tris, 192 mM Glycine, 20% (v/v) methanol, pH ~8.3.

Note: Methanol is not supplied but is required.

Bioactivity

Storage: Store 10X buffer at room temperature. At 10X, this buffer is stable for 24 months. Store at 4°C and use within 1 week once it has been diluted to 1X and methanol is added.

Directions for Use

To make 1L of 1X transfer buffer: Mix 100 ml of 10X transfer buffer, 200 ml of methanol, and 800 ml of ddH₂O and store at 4ºC for up to one week. This product supplies enough 10X material to make 10 liters of 1X solution. Generally, 20% methanol is recommended, however it may be beneficial to decrease methanol concentration to 5-10% for increased transfer efficiency of large, low abundancy proteins. CST recommends electrotransferring to 0.2 μm pore size nitrocellulose membranes at 70 volts for 2 hours.

Background

Tris-Glycine Transfer Buffer (10X) is a commonly used western blot buffer for the electrotransfer of proteins from SDS-PAGE gels to nitrocellulose or PVDF membranes. The formulation is based on the widely accepted Towbin transfer buffer (1) and is for use in tank (wet) transfer systems, the recommended system used by Cell Signaling Technology (CST) scientists.

注意事项：

Storage: Store 10X buffer at room temperature. At 10X, this buf-
fer is stable for 24 months. Store at 4°C and use within 1 week
once it has been diluted to 1X and methanol is added