SAM68 Antibody

• 产品编号：CST-12538S      品牌：CST       原厂货号：12538S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SAM68 Antibody能够检测内源性的SAM68总蛋白。该多克隆抗体是经由合成的围绕人 SAM68蛋白近羧基端的氨基酸肽段免疫动物而生产的。经过Protein A和亲和层析纯化。Src associated in mitosis 68 kDa (SAM68)是和RNA结合的RNA信号转导与激活(STAR)家族的成员之一（1,2）。SAM68是一个多功能蛋白在信号转导、细胞周期调控、转录以及选择性剪接中都发挥作用（1-3）。SAM68可作为支架蛋白来介导针对多种活化受体的刺激物的应激反应比如T细胞受体以及胰岛素受体，还可将多种信号转导途径的激活与转录后基因调控联系在一起（3）。SAM68是Src相关激酶和Erk1/2丝裂原活化蛋白激酶的磷酸化靶标。SAM68的磷酸化可调控其与RNA的亲和性以及像CD44 受体mRNA一样的RNA的选择性剪接（1,4）。许多SAM68的活动及其选择性剪接的靶标在脊髓性肌萎缩和肿瘤的发育和发展中都涉及到了，比如在前列腺癌和乳腺癌中SAM68被频繁地上调（1,5-8）。

1. Bielli, P. et al. (2011) Endocr Relat Cancer 18, R91-R102.
2. Lukong, K.E. and Richard, S. (2003) Biochim Biophys Acta 1653, 73-86.
3. Najib, S. et al. (2005) Cell Mol Life Sci 62, 36-43.
4. Matter, N. et al. (2002) Nature 420, 691-5.
5. Pedrotti, S. et al. (2010) EMBO J 29, 1235-47.
6. Busà, R. et al. (2007) Oncogene 26, 4372-82.
7. Rajan, P. et al. (2008) J Pathol 215, 67-77.
8. Song, L. et al. (2010) J Pathol 222, 227-37.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog, Pig, Guinea Pig, Horse

Specificity / Sensitivity

SAM68 Antibody recognizes endogenous levels of total SAM68 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SAM68 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Src associated in mitosis 68 kDa (SAM68) is a member of the signal transduction and activation of RNA (STAR) family of RNA binding proteins (1,2). SAM68 is a multi-functional protein with roles in signal transduction, cell cycle regulation, transcription, and alternative splicing (1-3). SAM68 acts as a scaffold protein mediating responses to various stimuli that activate receptors, such as the T-cell receptor and the insulin receptor, linking activation of various signal transduction pathways with post-transcriptional gene regulation (3). SAM68 is a target for phosphorylation by Src-related kinases and Erk1/2 mitogen-activated protein kinases. Phosphorylation of SAM68 regulates both its affinity for RNA and alternative splicing of RNAs like CD44 receptor mRNA (1,4). Many of the activities of SAM68 and its targets for alternative splicing have been implicated in the development and progression of spinal muscular atrophy and cancers such as prostate and breast carcinomas, where SAM68 is frequently upregulated (1,5-8).

1. Bielli, P. et al. (2011) Endocr Relat Cancer 18, R91-R102.
2. Lukong, K.E. and Richard, S. (2003) Biochim Biophys Acta 1653, 73-86.
3. Najib, S. et al. (2005) Cell Mol Life Sci 62, 36-43.
4. Matter, N. et al. (2002) Nature 420, 691-5.
5. Pedrotti, S. et al. (2010) EMBO J 29, 1235-47.
6. Busà, R. et al. (2007) Oncogene 26, 4372-82.
7. Rajan, P. et al. (2008) J Pathol 215, 67-77.
8. Song, L. et al. (2010) J Pathol 222, 227-37.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.