SignalSilence??γ-Catenin siRNA I (Mouse Specific)

• 产品编号：CST-12523S      品牌：CST       原厂货号：12523S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

CST的SignalSilence® γ-Catenin siRNA I (Mouse Specific)允许研究者采用RNA干扰的方法来特异性地抑制ADAM9的表达，这是一种通过去除细胞内双链RNA分子而使基因表达能够选择性沉默的方法。所有CST的基因沉默siRNA产品都经过内部的严格检测并且采用western方法证实可以减少蛋白表达。寡核苷酸的合成通过trityl分析逐个检测碱基以确保适当的耦合效率。Oligo随后经亲和层析纯化。采用质谱法进一步分析RNA双链的退火，以证实精确的双链构成。通过质谱法比较每批产品与以前的产品，以最大程度确保产品批次间的一致性。CST推荐细胞裂解前采用100 nM γ-Catenin siRNA I（鼠特异性）作用48-72小时。对于每一个转染步骤，遵循转染试剂商提供的产品使用步骤。如果使用中有任何问题，可与CST联系。每一个产品包含了100次转染需要的量，相当于每次转染siRNA的终浓度为100nM，24孔板的每孔总体积为300微升。γ-连环蛋白，又被称为盘状球蛋白，是信号转导分子的Armadillo家族成员，该家族包括β-连环蛋白和果蝇蛋白armadillo (1)。这一蛋白家族在Wnt信号转导中起作用，该信号转导在胚胎发育和肿瘤发生中有重要作用(2-3)。尽管两种脊椎动物蛋白β- 和γ-连环蛋白显示了序列的同源性，γ-连环蛋白与β-连环蛋白的作用似乎不同(1, 4-6)。γ-连环蛋白定位于桥粒和粘着连接处，这两个位点都是细胞间粘附，并与经典的和桥粒的钙粘素胞浆区结构域相互作用。γ- 或β-连环蛋白与α-连环蛋白的相互作用，桥粒斑蛋白和其他连接蛋白提供了细胞间连接和肌动蛋白以及中间丝细胞骨架的连接。对这一连接的维持和/或修饰对于控制细胞粘附和迁移有至关重要的作用(1)。γ-连环蛋白通过磷酸化修饰后影响粘附和β-连环蛋白依赖的转录(7)，通过糖基化修饰影响粘附作用(8)。最近的研究表明γ-连环蛋白通过对生长因子刺激的应答从而调节桥粒的粘附(9)。

1. Zhurinsky, J. et al. (2000) J. Cell Sci. 113 ( Pt 18), 3127-3139.
2. Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell Dev. Biol. 14, 59-88.
3. Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
4. Zhurinsky, J. et al. (2000) Mol. Cell Biol. 20, 4238-4252.
5. Charpentier, E. et al. (2000) J. Cell Biol. 149, 503-520.
6. Kolligs, F.T. et al. (2000) Genes Dev. 14, 1319-13131.
7. Miravet, S. et al. (2003) Mol. Cell Biol. 23, 7391-7402.
8. Hu, P. et al. (2006) J. Biol. Chem. 281, 12786-12791.
9. Yin, T. et al. (2005) J. Biol. Chem. 280, 40355-40363.

原厂资料：

Description

SignalSilence® γ-Catenin siRNA I (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit γ-catenin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM γ-Catenin siRNA I (Mouse Specific) 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. 
 Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Background

Also known as plakoglobin, γ-catenin is a member of the Armadillo family of signaling molecules, which includes β-catenin and the Drosophila protein armadillo (1). This family of proteins is involved in Wnt signaling, which is important in embryonic development and in tumorigenesis (2-3). Although the two vertebrate proteins β- and γ-catenin display sequence homology, γ-catenin likely plays a role distinct from that of β-catenin (1, 4-6). γ-catenin localizes to desmosomes and adherens junctions, both sites of intercellular adhesion, and interacts with the cytoplasmic domains of classical and desmosomal cadherins. Interaction of γ- or β-catenin with α-catenin, desmoplakin and other junction proteins provides a link between intercellular junctions and the actin and intermediate filament cytoskeleton. Maintenance and/or modification of this link is vital for control of cell adhesion and migration (1). γ-catenin is modified by phosphorylation, affecting both adhesion and β-catenin dependent transcription (7), and by and O-glycosylation, affecting adhesion (8). Recent evidence suggests that γ-catenin regulates desmosomal adhesion in response to growth factor stimulation (9).

1. Zhurinsky, J. et al. (2000) J. Cell Sci. 113 ( Pt 18), 3127-3139.
2. Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell Dev. Biol. 14, 59-88.
3. Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
4. Zhurinsky, J. et al. (2000) Mol. Cell Biol. 20, 4238-4252.
5. Charpentier, E. et al. (2000) J. Cell Biol. 149, 503-520.
6. Kolligs, F.T. et al. (2000) Genes Dev. 14, 1319-13131.
7. Miravet, S. et al. (2003) Mol. Cell Biol. 23, 7391-7402.
8. Hu, P. et al. (2006) J. Biol. Chem. 281, 12786-12791.
9. Yin, T. et al. (2005) J. Biol. Chem. 280, 40355-40363.

注意事项：

Storage:  γ -Catenin siRNA I (Mouse Specific) is supplied in
RNAse-free water. Aliquot and store at -20ºC