LC3B (D11) XP??Rabbit mAb?(Biotinylated)

• 产品编号：CST-12513S      品牌：CST       原厂货号：12513S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

LC3B (D11) XP^® Rabbit mAb (Biotinylated) 兔单抗能够识别内源性LC3B总蛋白。与LC3其他亚型会发生交叉反应。与LC3B的类型II发生强烈反应。与啮齿类的LC3B发生较微弱的反应。该单克隆抗体通过合成肽免疫动物制备，这种合成肽与LC3蛋白氨基末端周围残基相一致。该CST公司抗体在最佳条件下与生物素结合。此种生物素化的抗体与未结合生物素的LC3B (D11) XP® Rabbit mAb 兔单抗#3868具有同源交叉反应。自噬是自噬溶酶体对其包裹的细胞质内含物进行降解的一种分解代谢过程(1,2)。自噬过程一般是在营养匮乏的条件下被激活，但是也与一些生理过程有关，如发育、分化、神经变性、感染和癌症(3)。自噬微管相关蛋白的轻链3（LC3）最初被认定为是微管相关蛋白1A和1B的一个亚基（被称为MAP1LC3）（4），随后发现该蛋白所包含的与酵母蛋白类似的Apg8/Aut7/Cvt5对于自噬起着至关重要的作用（5）。三种人类LC3亚型（LC3A、LC3B和LC3C）在自噬过程中翻译后被修饰（6-9）。随着合成后LC3羧基末端迅速裂解，产生了胞浆的LC3-I 型。在自噬过程中，通过一个涉及Atg7和Agt3的类泛素体系，LC3-I被脂质化为LC3-II，从而将LC3与自噬小泡联系起来（6-10）。自噬体中LC3的存在，及其向低迁移形式的LC3-II的转化被作为自噬发生的“指示器”（11）。

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot. Cell 1, 11-21.
2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18.
3. Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88.
4. Mann, S.S. and Hammarback, J.A. (1994) J. Biol. Chem. 269, 11492-97.
5. Lang, T. et al. (1998) EMBO J. 17, 3597-607.
6. Kabeya, Y. et al. (2000) EMBO J. 19, 5720-28.
7. He, H. et al. (2003) J. Biol. Chem. 278, 29278-87.
8. Tanida, I. et al. (2004) J. Biol. Chem. 279, 47704-10.
9. Wu, J. et al. (2006) Biochem. Biophys. Res. Commun. 339, 437-42.
10. Ichimura, Y. et al. (2000) Nature 408, 488-92.
11. Kabeya, Y. et al. (2004) J. Cell Sci. 117, 2805-12.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Bovine, Pig

Specificity / Sensitivity

LC3B (D11) XP^® Rabbit mAb (Biotinylated) detects endogenous levels of total LC3B protein. Cross-reactivity may occur with other LC3 isoforms. Stronger reactivity is observed with the type II form of LC3B. Weaker reactivity is observed with rodent LC3B.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of LC3B.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated LC3B (D11) XP^® Rabbit mAb #3868.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11).

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot. Cell 1, 11-21.
2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18.
3. Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88.
4. Mann, S.S. and Hammarback, J.A. (1994) J. Biol. Chem. 269, 11492-97.
5. Lang, T. et al. (1998) EMBO J. 17, 3597-607.
6. Kabeya, Y. et al. (2000) EMBO J. 19, 5720-28.
7. He, H. et al. (2003) J. Biol. Chem. 278, 29278-87.
8. Tanida, I. et al. (2004) J. Biol. Chem. 279, 47704-10.
9. Wu, J. et al. (2006) Biochem. Biophys. Res. Commun. 339, 437-42.
10. Ichimura, Y. et al. (2000) Nature 408, 488-92.
11. Kabeya, Y. et al. (2004) J. Cell Sci. 117, 2805-12.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50%
glycerol. Store at –20°C. Do not aliquot the antibodies.