Caveolin-1 (D46G3) XP??Rabbit mAb?(HRP Conjugate)

• 产品编号：CST-12506S      品牌：CST       原厂货号：12506S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Caveolin-1 (D46G3) XP® Rabbit mAb兔单抗 (HRP接合)能够检测内源性的caveolin-1总蛋白。该单克隆抗体是经由合成的围绕人caveolin-1蛋白Glu20位点的氨基酸肽段免疫动物而生产的。该CST抗体通过其氨基基团与辣根过氧化物酶 (HRP)的羧基进行偶联。它与未偶联的 Caveolin-1 (D46G3) XP® Rabbit mAb 兔单抗#3267具有相同的种属交叉反应性。21-24kDa的整合蛋白，caveolins，是胆固醇/神经鞘脂富集的细胞膜微区域小窝的核心结构。caveolin家族的三个成员（caveolin-1，-2，-3）已经识别出来，它们在不同组织中分布不同。Caveolin形成异源和同源寡聚体，与胆固醇和其它脂质相互作用（1）。Caveolin参与多种生物功能，包括囊泡转运、胆固醇稳态、细胞黏附、凋亡，在神经退行性疾病中也有作用（2）。Caveolin也与多种信号分子相互作用，如Gα亚基、酪氨酸激酶受体、PKCs、Scr家族酪氨酸激酶和eNOS（1，2）。目前认为Caveolin是一个整合信号传导的脚手架蛋白。其Tyr14的磷酸化对其与包含SH2或PTB结构域的接头蛋白如GRB7的结合至关重要（3-5）。Ser80的磷酸化调节caveolin结合到内质网膜并进入分泌途径（6）。

1. Okamoto, T. et al. (1998) J Biol Chem 273, 5419-22.
2. Smart, E.J. et al. (1999) Mol Cell Biol 19, 7289-304.
3. Nomura, R. et al. (1999) Mol. Biol. Cell 10, 975-986.
4. Volonte, D. et al. (2001) J. Biol. Chem. 276, 8094-8103.
5. Lee, H. et al. (2000) Mol Endocrinol 14, 1750-75.
6. Schlegel, A. et al. (2001) J Biol Chem 276, 4398-408.

原厂资料：

Specificity / Sensitivity

Caveolin-1 (D46G3) XP® Rabbit mAb (HRP Conjugate) detects endogenous levels of total caveolin-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu20 of human caveolin-1 protein.

Description

This Cell Signaling Technology® antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Caveolin-1 (D46G3) XP® Rabbit mAb #3267.

Background

The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions. Caveolins form hetero- and homo-oligomers that interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion, and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins interact with multiple signaling molecules such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases, and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6).

1. Okamoto, T. et al. (1998) J Biol Chem 273, 5419-22.
2. Smart, E.J. et al. (1999) Mol Cell Biol 19, 7289-304.
3. Nomura, R. et al. (1999) Mol. Biol. Cell 10, 975-986.
4. Volonte, D. et al. (2001) J. Biol. Chem. 276, 8094-8103.
5. Lee, H. et al. (2000) Mol Endocrinol 14, 1750-75.
6. Schlegel, A. et al. (2001) J Biol Chem 276, 4398-408.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.