Tris Buffered Saline (TBS-10X)

• 产品编号：CST-12498S      品牌：CST       原厂货号：12498S
• 产品分类：生化试剂 > 生物缓冲液 > TBS
• 应用分类：

描述：

Tris buffered saline (TBS) solution用作在 western blotting中用作封闭缓冲液。Tween 20^®不能加入封闭缓冲液，因为它可以自发显影,增加非特异性背景。封闭后, Tween 20^®可以重新加入稀释缓冲。Chemliluminescent western blotting在封闭时可以加入需要Tween 20^®。产品运输和储存在室温下。1 x配方:137 mM氯化钠,20 mM Tris。pH值7.6。注意:本产品不包含Tween 20^®detergent。

储存：室温储存10 X溶液，稳定24个月。稀释成1X后保存1周。

用双蒸水稀释Tris Buffered Saline (TBS-10X)至1 X。本产品供应足够的材料配制成10升1 x的溶液。

原厂资料：

Description

Tris buffered saline (TBS) solution for use as the blocking buffer diluent during fluorescent western blotting. Tween 20^® detergent cannot be present in the blocking buffer because it can auto-fluoresce and increase non-specific background. After the blocking step, Tween 20^® can be re-introduced to subsequent diluent buffers. Chemliluminescent western blotting does not require Tween 20^® omission during blocking. Product is shipped and stored at room temperature.

1X Formulation: 137 mM Sodium Chloride, 20 mM Tris. Supplied at pH 7.6.

NOTE: This product does not contain Tween 20^® detergent.

Bioactivity

Storage: Store 10X buffer at room temperature. At 10X, this buffer is stable for 24 months. Use within 1 week once it has been diluted to 1X.

Directions for Use

Dilute Tris Buffered Saline (TBS-10X) to a 1X solution using ddH₂O. This product supplies enough 10X material to make 10 liters of 1X solution.

注意事项：

Storage: Store 10X buffer at room temperature. At 10X, this
buffer is stable for 24 months. Use within 1 week once it has
been diluted to 1X.