PRMT4/CARM1 (3H2) Mouse mAb

• 产品编号：CST-12495S      品牌：CST       原厂货号：12495S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PRMT4/CARM1 (3H2) Mouse mAb鼠单抗能够检测内源性PRMT4/CARM1 总蛋白水平。该单克隆抗体经是采用合成的人CARM1重组蛋白免疫动物而生产的。Protein arginine N-methyltransferase 4 (PRMT4)是蛋白精氨酸甲基转移酶(PRMT)蛋白家族的一个成员，它以S-腺苷-甲硫氨酸(S-adenosylmethionine,AdoMet)为甲基供体，把甲基转移到蛋白精氨酸胍基的氮原子上 (1)。尽管所有的PRMT蛋白都能催化单甲基精氨酸的形成，但Type I PRMTs (PRMT1, 3, 4, 和 6)能够添加另一个甲基基团从而产生一个不对称的二甲基化精氨酸，而Type II PRMTs (PRMT 5 and 7)则能产生一个对称的二甲基精氨酸（1）。单甲基化精氨酸，而非二甲基化精氨酸可以通过由类似PADI4这样的酶催化的脱氨基作用来转变为瓜氨酸（2）。大部分PRMT包括PRMT1，能够甲基化在富含甘氨酸 - 精氨酸（GAR）的蛋白结构域内发现的精氨酸残基，例如RGG, RG, 和 RXR重复序列中 (1)。然而，PRMT4/CARM1 和 PRMT5可以使PGM (proline-, glycine-, methionine-rich) 结构中精氨酸甲基化(3)。PRMT1能够甲基化组蛋白H4的Arg3 位点，并且能够与p300/CBP协同作用通过核受体蛋白来增强转录激活（4-6）。此外，PRMT1能够甲基化组非组蛋白，包括孤儿核受体HNF4（6）、不均一核糖核蛋白（hnRNP）颗粒的组分（7）、RNA结合蛋白Sam68（8）、白介素增强子结合因子3（ILF3）（9）以及干扰素-α 和 β受体（10）。这些相互作用暗示着PRMT1在转录调控、mRNA加工和信号转导还有一些其他的功能。可变RNA剪接会产生3种氨基末端区域不一样的酶促激活PMRT1异构体（11）。PRMT1根据不同的细胞类型会定位在和或者细胞质中（12,13），同时会在许多不同的蛋白复合物中出现。ILF3、TIS21以及白血病相关性BTG1蛋白能够结合PRMT1从而条龙其甲基转移酶活性（9,14）。

1. Bedford, M.T. and Richard, S. (2005) Mol. Cell 18, 263-272.
2. Wang, Y. et al. (2004) Science 306, 279-283.
3. Cheng, D. et al. (2007) Mol. Cell 25, 71-83.
4. Wang, H. et al. (2001) Science 293, 853-857.
5. Strahl, B.D. et al. (2001) Curr. Biol. 11, 996-1000.
6. Barrero, M.J. and Malik, S. (2006) Mol. Cell 24, 233-243.
7. Nichols, R.C. et al. (2000) Exp. Cell Res. 256, 522-532.
8. Côté, J. et al. (2003) Mol. Biol. Cell 14, 274-287.
9. Tang, J. et al. (2000) J. Biol. Chem. 275, 19866-19876.
10. Abramovich, C. et al. (1997) EMBO J. 16, 260-266.
11. Scorilas, A. et al. (2000) Biochem. Biophys. Res. Commun. 278, 349-359.
12. Frankel, A. et al. (2002) J. Biol. Chem. 277, 3537-3543.
13. Herrmann, F. et al. (2005) J. Biol. Chem. 280, 38005-38010.
14. Lin, W.J. et al. (1996) J. Biol. Chem. 271, 15034-15044.

原厂资料：

Specificity / Sensitivity

PRMT4/CARM1 (3H2) Mouse mAb recognizes endogenous levels of total PRMT4/CARM1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the human CARM1 protein.

Background

Protein arginine N-methyltransferase 1 (PRMT1) is a member of the protein arginine N-methyltransferase (PRMT) family of proteins that catalyze the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine (1). Though all PRMT proteins catalyze the formation of mono-methyl arginine, Type I PRMTs (PRMT1, 3, 4, and 6) add an additional methyl group to produce an asymmetric di-methyl arginine while Type II PRMTs (PRMT 5 and 7) produce symmetric di-methyl arginine (1). Mono-methyl arginine, but not di-methyl arginine, can be converted to citrulline through deimination catalyzed by enzymes such as PADI4 (2). Most PRMTs, including PRMT1, methylate arginine residues found within glycine-arginine rich (GAR) protein domains, such as RGG, RG, and RXR repeats (1). However, PRMT4/CARM1 and PRMT5 methylate arginine residues within PGM (proline-, glycine-, methionine-rich) motifs (3). PRMT1 methylates Arg3 of histone H4 and cooperates synergistically with p300/CBP to enhance transcriptional activation by nuclear receptor proteins (4-6). In addition, PRMT1 methylates many non-histone proteins, including the orphan nuclear receptor HNF4 (6), components of the heterogeneous nuclear ribonucleoprotein (hnRNP) particle (7), the RNA binding protein Sam68 (8), interleukin enhancer-binding factor 3 (ILF3) (9) and interferon-α and β receptors (10). These interactions suggest additional functions in transcriptional regulation, mRNA processing and signal transduction. Alternative mRNA splicing produces three enzymatically active PMRT1 isoforms that differ in their amino-terminal regions (11). PRMT1 is localized to the nucleus or cytoplasm, depending on cell type (12,13), and appears in many distinct protein complexes. ILF3, TIS21 and the leukemia-associated BTG1 proteins bind PRMT1 to regulate its methyltransferase activity (9,14).

1. Bedford, M.T. and Richard, S. (2005) Mol. Cell 18, 263-272.
2. Wang, Y. et al. (2004) Science 306, 279-283.
3. Cheng, D. et al. (2007) Mol. Cell 25, 71-83.
4. Wang, H. et al. (2001) Science 293, 853-857.
5. Strahl, B.D. et al. (2001) Curr. Biol. 11, 996-1000.
6. Barrero, M.J. and Malik, S. (2006) Mol. Cell 24, 233-243.
7. Nichols, R.C. et al. (2000) Exp. Cell Res. 256, 522-532.
8. Côté, J. et al. (2003) Mol. Biol. Cell 14, 274-287.
9. Tang, J. et al. (2000) J. Biol. Chem. 275, 19866-19876.
10. Abramovich, C. et al. (1997) EMBO J. 16, 260-266.
11. Scorilas, A. et al. (2000) Biochem. Biophys. Res. Commun. 278, 349-359.
12. Frankel, A. et al. (2002) J. Biol. Chem. 277, 3537-3543.
13. Herrmann, F. et al. (2005) J. Biol. Chem. 280, 38005-38010.
14. Lin, W.J. et al. (1996) J. Biol. Chem. 271, 15034-15044.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.