HIRA (D6O8L) Rabbit mAb

• 产品编号：CST-12463S      品牌：CST       原厂货号：12463S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HIRA (D6O8L) Rabbit mAb兔单抗能够检测内源性HIRA总蛋白水平。该单克隆抗体经由人HIRA蛋白羧基端的重组蛋白免疫动物而生产的。Histone cell cycle regulation defective homolog A (HIRA)，也被称为TUP1-like enhancer of split protein 1 (TUPLE1)，它是酵母中HIR1和HIR2转录阻遏子蛋白在哺乳动物中的同源物（1）。在细胞核中，HIRA能够与UBN1, CABIN以及 ASF1A相互作用形成进化上保守的HUCA组蛋白伴侣复合物从而以一种DNA复制非依赖性的方式将组蛋白H3.3的变异体沉积到染色质中（2）。HIRA是组蛋白H3.3沉积到基因的转录开始位点所需要的，在这个位点上组蛋白H3.3能够协同促进核小体的去稳定作用，并且促进转录激活（3-5）。组蛋白H3.3同时与基因沉默有关，它被认为可掺入到转录性失活的基因组区域（5-7）。而另外一种含有ATRX 和 DAXX的组蛋白伴侣复合物则可以促进H3.3掺入到异染色质中（如，H3.3的端粒掺入）。HIRA是细胞衰老过程中组蛋白H3.3掺入以及衰老相关异染色质基因座（SAHF）的形成所需要的（5-8）。在小鼠胚胎发育过程HIRA的表达无所不在（9）。在成年小鼠内，HIRA在肾，骨骼肌以及胰腺中高水平表达，但是在心脏、肺、胎盘、大脑以及肝中呈低水平表达（9）。人染色体22q11.2区的HIRA基因缺失一个拷贝，这是DiGeorge综合症病人的一个普遍特征。HIRA蛋白的生产不足可能会破坏正常的胚胎发育（9）。

1. Lamour, V. et al. (1995) Hum Mol Genet 4, 791-9.
2. Rai, T.S. et al. (2011) Mol Cell Biol 31, 4107-18.
3. Jin, C. et al. (2009) Nat Genet 41, 941-5.
4. Jin, C. and Felsenfeld, G. (2007) Genes Dev 21, 1519-29.
5. Goldberg, A.D. et al. (2010) Cell 140, 678-91.
6. Wong, L.H. et al. (2010) Genome Res 20, 351-60.
7. Wong, L.H. et al. (2009) Genome Res 19, 404-14.
8. Zhang, R. et al. (2007) Mol Cell Biol 27, 2343-58.
9. Wilming, L.G. et al. (1997) Hum Mol Genet 6, 247-58.

原厂资料：

Specificity / Sensitivity

HIRA (D6O8L) Rabbit mAb recognizes endogenous levels of total HIRA protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human HIRA protein.

Background

Histone cell cycle regulation defective homolog A (HIRA), also known as TUP1-like enhancer of split protein 1 (TUPLE1), is the mammalian homolog of the yeast HIR1 and HIR2 transcriptional repressor proteins (1). HIRA interacts with UBN1, CABIN, and ASF1A in the cell nucleus to form the evolutionarily conserved HUCA histone chaperone complex that deposits the variant histone H3.3 into chromatin in a DNA-replication independent manner (2). HIRA is required for deposition of histone H3.3 at the transcription start sites of genes, where incorporation of histone H3.3 facilitates nucleosome destabilization and contributes to transcriptional activation (3-5). Histone H3.3 is also linked to gene silencing and is incorporated into regions of the genome thought to be transcriptionally inactive (5-7). While some incorporation of H3.3 into heterochromatin is facilitated by an additional histone chaperone complex containing ATRX and DAXX (ie. telomeric incorporation of H3.3), HIRA is required for incorporation of histone H3.3 and formation of senescence-associated heterochromatin foci (SAHF) during cellular senescence (5-8). HIRA is ubiquitously expressed during mouse embryonic development (9). In the adult mouse, HIRA is expressed at high levels in the kidney, skeletal muscle, and pancreas, but it is expressed at lower levels in the heart, lung, placenta, brain, and liver (9). A missing copy of the HIRA gene on human chromosome region 22q11.2 is a common characteristic of DiGeorge syndrome patients and insufficient production of the HIRA protein may disrupt normal embryonic development (9).

1. Lamour, V. et al. (1995) Hum Mol Genet 4, 791-9.
2. Rai, T.S. et al. (2011) Mol Cell Biol 31, 4107-18.
3. Jin, C. et al. (2009) Nat Genet 41, 941-5.
4. Jin, C. and Felsenfeld, G. (2007) Genes Dev 21, 1519-29.
5. Goldberg, A.D. et al. (2010) Cell 140, 678-91.
6. Wong, L.H. et al. (2010) Genome Res 20, 351-60.
7. Wong, L.H. et al. (2009) Genome Res 19, 404-14.
8. Zhang, R. et al. (2007) Mol Cell Biol 27, 2343-58.
9. Wilming, L.G. et al. (1997) Hum Mol Genet 6, 247-58.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.