BRE (D8Q1J) Rabbit mAb

• 产品编号：CST-12457S      品牌：CST       原厂货号：12457S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

BRE (D8Q1J)Rabbit mAb可以识别内源性BRE总蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人BRE蛋白的150谷氨酰胺及其邻近氨基酸残基序列一致。乳腺癌易感基因，BRCA1，编码一种E3泛素连接酶，BRCA1通过调控DNA损伤反应和DNA修复维持基因组的稳定性。BRCA1会和其他DNA损伤蛋白结合形成至少三种不同的复合物（BRCA1A,B和C），这种结合对于调控BRCA1的功能是至关重要的。BRCA1-Rap80 复合物 (BRCA1 A复合物)，包括Rap80, BRCC36, BRCC45, Abraxas, 和MERIT40/NBA1,调控G2/M周期检验点（1,2）。MERIT40和BRE能维持BRCA1 A复合物和细胞质BRISC复合物的稳定性，BRISC复合物包括BRCC36 和ABRO1而不包括BRCA1 (3)。研究人员证实了BRE的表达水平与乳腺癌患者的存活率有关（4），在急性骨髓性白血病中(AML)它可能预示着一个一个好的结果（5,6）。研究也指出在人肝癌组织中BRE是过表达的（7），并且BRE的过表达可能会引发抗凋亡通路并促进肿瘤生长（7,8）。

1. Ohta, T. et al. (2011) FEBS Lett 585, 2836-44.
2. Huen, M.S. et al. (2010) Nat Rev Mol Cell Biol 11, 138-48.
3. Hu, X. et al. (2011) J Biol Chem 286, 11734-45.
4. Noordermeer, S.M. et al. (2012) Breast Cancer Res Treat 135, 125-33.
5. Noordermeer, S.M. et al. (2011) Blood 118, 5613-21.
6. Balgobind, B.V. et al. (2010) Leukemia 24, 2048-55.
7. Chan, B.C. et al. (2008) Oncogene 27, 1208-17.
8. Chui, Y.L. et al. (2010) Biochem Biophys Res Commun 391, 1522-5.

原厂资料：

Specificity / Sensitivity

BRE (D8Q1J) Rabbit mAb recognizes endogenous levels of total BRE protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln150 of human BRE protein.

Background

The breast cancer susceptibility gene, BRCA1, codes for an E3 ubiquitin ligase that functions in the maintenance of genome stability through regulation of the DNA damage response and DNA repair. BRCA1 protein forms at least three distinct complexes (BRCA1 A, B, and C) with other DNA repair proteins, and these interactions are vital for regulation of BRCA1 function. The BRCA1 A complex includes Rap80, BRCC36, Abraxas, MERIT40/NBA1, and BRE/BRCC45 and functions in G2/M phase checkpoint control (reviewed in 1,2). MERIT40 and BRE maintain the stability of both the BRCA1 A complex and the cytoplasmic BRISC complex, which contains BRCC36 and ABRO1 but not BRCA1 (3). 
 Researchers have shown that the expression level of BRE is related to patient survival in breast cancer (4), and it may predict a favorable outcome in acute myeloid leukemia (AML) (5,6). Studies have also shown that BRE is overexpressed in human hepatocellular carcinoma (7) and that overexpression of BRE can cause resistance to apoptotic signaling and promote tumor growth (7,8).

1. Ohta, T. et al. (2011) FEBS Lett 585, 2836-44.
2. Huen, M.S. et al. (2010) Nat Rev Mol Cell Biol 11, 138-48.
3. Hu, X. et al. (2011) J Biol Chem 286, 11734-45.
4. Noordermeer, S.M. et al. (2012) Breast Cancer Res Treat 135, 125-33.
5. Noordermeer, S.M. et al. (2011) Blood 118, 5613-21.
6. Balgobind, B.V. et al. (2010) Leukemia 24, 2048-55.
7. Chan, B.C. et al. (2008) Oncogene 27, 1208-17.
8. Chui, Y.L. et al. (2010) Biochem Biophys Res Commun 391, 1522-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody