SignalSilence? MKK7 siRNA I (Mouse Specific)

• 产品编号：CST-12442S      品牌：CST       原厂货号：12442S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence® MKK7 siRNA I（小鼠特异性）可以抑制小鼠和大鼠的MKK7的表达。来自Cell Signaling Technology (CST)的SignalSilence^® MKK7 siRNA I (Mouse Specific) 可以帮助研究者通过RNA干扰特异性地抑制MKK7的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^® MKK7 siRNA I (Mouse Specific) 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。每小瓶可供100次转染，每次转染量相当于在转染24孔板时，每孔总体积为300μl培养基中siRNA的终浓度为100nM。MKK7是一种MAPKK，它是JNK/SAPK通路的特异性活化因子(1,2)。MKK7能被TNFα以及其他环境压力显著激活，然而能激活 p38、JNK/SAPK通路的SEK1/MKK4却不能被TNFα所激活(2)。对MAPKK家族成员活化环的序列比对表明，MKK7蛋白的Ser271和Thr275位点是潜在的磷酸化位点，这两个位点对激酶的活性十分重要。

1. Tournier, C. et al. (1999) Mol. Cell. Biol. 19, 1569-1581.
2. Moriguchi, T. et al. (1997) EMBO J. 16, 7045-7053.

原厂资料：

omology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

SignalSilence® MKK7 siRNA I (Mouse Specific) inhibits mouse and rat MKK7 expression.

Description

SignalSilence® MKK7 siRNA I (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit MKK7 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® MKK7 siRNA I (Mouse Specific) 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. 
 Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Background

MKK7 is a MAP kinase kinase that serves as a specific activator of the SAPK/JNK pathway (1,2). MKK7 is strongly activated by TNF-α, as well as other environmental stresses, whereas SEK1/MKK4, which activates both p38 and SAPK/JNK pathways, is not activated by TNF-α (2). Sequence alignment of the activation loop of the MAP kinase kinase family members indicates that Ser271 and Thr275 are potential phosphorylation sites that are crucial for the kinase acivity.

1. Tournier, C. et al. (1999) Mol. Cell. Biol. 19, 1569-1581.
2. Moriguchi, T. et al. (1997) EMBO J. 16, 7045-7053.

注意事项：

Storage: MKK7 siRNA I (Mouse Specific) is supplied in RNAse-
free water. Aliquot and store at -20ºC.