S5a/PSMD4 (D20B2) Rabbit mAb

• 产品编号：CST-12441S      品牌：CST       原厂货号：12441S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

S5a/PSMD4 (D20B2) Rabbit mAb兔单抗识别内源性的S5a/PSMD4蛋白。此单克隆抗体经合成与人S5a/PSMD4蛋白羧基末端邻近氨基酸残基序列已知的肽段，免疫动物产生。S5a (PSMD4)是一种19S调节蛋白酶体复合物的亚基，在泛素蛋白靶向作用和降解中起作用(1)。S5a包括两种泛素结合模序(UIM)，通过疏水作用结合多泛素链(2,3)。除外泛素，S5a的UIM显示了与多种蛋白中泛素样结构域的高亲和力。S5a与这些种类蛋白直接结合，并介导它们对于蛋白酶体降解的靶向作用(4,5)。

1. Deveraux, Q. et al. (1995) J. Biol. Chem. 270, 23726-23729.
2. Young, P. et al. (1998) J. Biol. Chem. 273, 5461-5467.
3. Beal, R. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 861-866.
4. Fujiwara, K. et al. (2004) J. Biol. Chem. 279, 4760-4767.
5. Sakata, E. et al. (2003) EMBO Rep. 4, 301-306.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Xenopus, Zebrafish, Bovine, Dog, Pig, Horse

Specificity / Sensitivity

S5a/PSMD4 (D20B2) Rabbit mAb recognizes endogenous levels of total S5a/PSMD4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human S5a/PSMD4 protein.

ackground

S5a (PSMD4) is a subunit of the 19S regulatory proteasome complex functioning in ubiquitinated-protein targeting and degradation (1). S5a contains two polyubiquitin binding motifs (UIM) that bind multiubiquitin chains by hydrophobic interaction (2,3). In addition to ubiquitin, the UIM of S5a shows high affinity to a ubiquitin-like domain present in many proteins. S5a binds to these types of proteins directly and mediates their targeting to the proteasome for degradation (4,5).

1. Deveraux, Q. et al. (1995) J. Biol. Chem. 270, 23726-23729.
2. Young, P. et al. (1998) J. Biol. Chem. 273, 5461-5467.
3. Beal, R. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 861-866.
4. Fujiwara, K. et al. (2004) J. Biol. Chem. 279, 4760-4767.
5. Sakata, E. et al. (2003) EMBO Rep. 4, 301-306.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.