SignalSilence? MAML1 siRNA II

• 产品编号：CST-12440S      品牌：CST       原厂货号：12440S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence^® MAML1 siRNA II可以抑制人和猴的MAML1基因表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。Mastermind-like (MAML)家族蛋白是果蝇Mastermind的同源物。该家族在哺乳动物中有3个成员：MAML1，MAML2,和MAML3 (1,2)。MAML蛋白与Notch（ICN）的细胞内结构域和转录因子CSL (RBP-Jκ)结合形成复合物调控Notch靶基因表达（3-5）。MAML1可以与肌肉细胞增强因子2C（MEF2C）结合调控肌细胞生成（6）。MAML2经常发现在粘表皮样癌病人和乳头状淋巴性腺囊瘤体内，与粘表皮样癌转位基因1（MECT1,也被称为WAMTP1或TORC1）融合（7）。

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

SignalSilence^® MAML1 siRNA II inhibits human and monkey MAML1 expression.

Description

SignalSilence® MAML1 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit MAML1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® MAML1 siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. 
 Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

ackground

Mastermind-like (MAML) family of proteins are homologs of Drosophila Mastermind. The family is composed of three members in mammals: MAML1, MAML2, and MAML3 (1,2). MAML proteins form complexes with the intracellular domain of Notch (ICN) and the transcription factor CSL (RBP-Jκ) to regulate Notch target gene expression (3-5). MAML1 also interacts with myocyte enhancer factor 2C (MEF2C) to regulate myogenesis (6). MAML2 is frequently found to be fused with Mucoepidermoid carcinoma translocated gene 1 (MECT1, also know as WAMTP1 or TORC1) in patients with mucoepidermoid carcinomas and Warthin's tumors (7).

注意事项：

Storage: MAML1 siRNA II is supplied in RNAse-free water.
Aliquot and store at -20ºC