Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb

• 产品编号：CST-12438S      品牌：CST       原厂货号：12438S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb兔单抗仅能够检测发生了34位苏氨酸磷酸化的内源性的DARPP-32蛋白。该单克隆抗体是由合成的人源DARPP-32蛋白苏氨酸(34位)肽段免疫动物而制备的。DARPP-32（多巴胺和环磷酸腺苷调节的磷酸化蛋白，相对分子质量32000）是一种在纹状体中型棘神经元高度富集的胞浆蛋白（1）。这是一个双功能的信号分子，控制丝氨酸/苏氨酸激酶和丝氨酸/苏氨酸磷酸酶的活性（2）。多巴胺通过刺激多巴胺D1受体和激活PKA来磷酸化DARPP-32。PKA磷酸化DARPP-32 Thr34，将其转换成一种蛋白磷酸酶1抑制剂（1）。DARPP-32被细胞周期蛋白依赖性激酶5（CDK5）在Thr75磷酸化后，被转换成PKA的抑制剂（2）。DARPP-32基因靶向敲除小鼠表现出生化，电生理和行为的改变（3）。

1. Nishi, A. et al. (1997) J. Neurosci. 17, 8147-8155.
2. Bibb, J.A. et al. (1999) Nature 402, 669-671.
3. Fienberg, A.A. et al. (1998) Science 281, 838-842.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb detects endogenous levels of DARPP-32 only when phosphorylated at Thr34.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr34 of human DARPP-32 protein.

Background

DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3).

1. Nishi, A. et al. (1997) J. Neurosci. 17, 8147-8155.
2. Bibb, J.A. et al. (1999) Nature 402, 669-671.
3. Fienberg, A.A. et al. (1998) Science 281, 838-842.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.