FIP200 (D10D11) Rabbit mAb

• 产品编号：CST-12436S      品牌：CST       原厂货号：12436S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FIP200 (D10D11) Rabbit mAb 兔单抗能识别内源性的FIP200总蛋白。该单克隆抗体是采用合成的人FIP200蛋白羧基末端附近的残基相对应的肽段免疫动物制备。FIP200（FAK家族激酶互作蛋白，200 kDa）与酪氨酸激酶Pyk2是在双杂交筛选时被发现的，并且 FIP200 能够抑制Pyk2激酶及其相关家族成员的活性（1）。随后，又在多重耐药筛选中发现FIP200，并被命名为RB1CC1，这是因为它能够诱导细胞毒性应激和调节RB1表达（2）。FIP200的功能与细胞凋亡、细胞周期进程、细胞生长和迁移相联系（回顾3）。最近又发现，FIP200能与ULK1相互作用，并且是自噬体形成的重要部分（4）。FIP200 与受mTOR调节的Atg13都是ULK1复合物的一部分，也是饥饿诱导的自噬所必需的（5-7）。

1. Ueda, H. et al. (2000) J Cell Biol 149, 423-30.
2. Chano, T. et al. (2002) Oncogene 21, 1295-8.
3. Gan, B. and Guan, J.L. (2008) Cell Signal 20, 787-94.
4. Hara, T. et al. (2008) J Cell Biol 181, 497-510.
5. Hosokawa, N. et al. (2009) Mol Biol Cell 20, 1981-91.
6. Jung, C.H. et al. (2009) Mol Biol Cell 20, 1992-2003.
7. Ganley, I.G. et al. (2009) J Biol Chem 284, 12297-305.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

FIP200 (D10D11) Rabbit mAb recognizes endogenous levels of total FIP200 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FIP200 protein.

Background

FIP200 (FAK family kinase-interacting protein of 200 kDa) was identified in a two-hybrid screen with the tyrosine kinase Pyk2 and can inhibit Pyk2 kinase activity as well as related family members (1). FIP200 was later independently identified in a multi-drug resistance screen and named RB1CC1 (RB1-inducible coiled-coil 1) due to its induction by cytotoxic stress and RB1 expression regulation (2). FIP200 function has been linked to apoptosis, cell cycle progression, cell growth, and migration (reviewed in 3). FIP200 has also recently been shown to interact with ULK1 and is required for autophagosome formation (4). FIP200 is part of an ULK1 complex along with Atg13 that is regulated by mTOR and is required for starvation induced autophagy (5-7).

1. Ueda, H. et al. (2000) J Cell Biol 149, 423-30.
2. Chano, T. et al. (2002) Oncogene 21, 1295-8.
3. Gan, B. and Guan, J.L. (2008) Cell Signal 20, 787-94.
4. Hara, T. et al. (2008) J Cell Biol 181, 497-510.
5. Hosokawa, N. et al. (2009) Mol Biol Cell 20, 1981-91.
6. Jung, C.H. et al. (2009) Mol Biol Cell 20, 1992-2003.
7. Ganley, I.G. et al. (2009) J Biol Chem 284, 12297-305.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.