Keratin 17/19 (D4G2) XP? Rabbit mAb

• 产品编号：CST-12434P      品牌：Cell Signaling Technology       原厂货号：12434P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Keratin 17/19 (D4G2) XP^® Rabbit mAb识别内源性的keratin17和keratin19蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人keratin 17和keratin 19蛋白氨基末端邻近氨基酸序列一致。角蛋白（细胞角蛋白）是中间丝蛋白主要在上皮细胞中表达。角蛋白的异二聚体由一个酸性角蛋白（或type I角蛋白，角蛋白9-23或）和一个碱性角蛋白（type II角蛋白，角蛋白1-8）聚合形成中间丝（1.2）。角蛋白的亚型在组织-和分化的特异性表达，这使他们可做为有用的生物标志（1）。研究显示角蛋白基因突变与肌肤失调，肝脏和胰腺疾病，以及肠道炎性疾病相关（3-6）。Keratin 17涉及到损伤修复和细胞生长两个都要求迅速的细胞骨架重构的过程（7）。keratin 17缺陷的角质细胞Akt/mTOR信号通路异常不能激活翻译，细胞大小或生长；这些细胞14-3-3σ蛋白位置会发生异常。由于14-3-3σ通常和keratin17结合，这些结果提示Akt/mTOR信号会导致细胞质中14-3-3σ与keratin17分离开，而它们的结合对于翻译和细胞生长是必须的。Keratin17的Ser44磷酸化可能会提供一个14-3-3σ的结合位点（8）。

1. Moll, R. et al. (1982) Cell 31, 11-24.
2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
7. Paladini, R.D. et al. (1996) J Cell Biol 132, 381-97.
8. Kim, S. et al. (2006) Nature 441, 362-5.

原厂资料：

pecificity / Sensitivity

Keratin 17/19 (D4G2) XP^® Rabbit mAb detects endogenous levels of keratin 17 and keratin 19 proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids near the amino terminus of human keratin 17 and human keratin 19 proteins.

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).Keratin 17 is involved in wound healing and cell growth, two processes that require rapid cytoskeletal remodeling (7). Keratinocytes deficient in keratin 17 exhibit abnormal Akt/mTOR signaling and fail to produce an increase in translation, cell size, or growth; these cells also exhibit abnormal 14-3-3σ localization. As 14-3-3σ typically associates with keratin 17, these results imply that Akt/mTOR signaling results in sequestration of 14-3-3σ with keratin 17 in the cytosol, which is required for translation and cell growth. Phosphorylation of keratin 17 on Ser44 may provide a docking site for 14-3-3σ binding (8).

1. Moll, R. et al. (1982) Cell 31, 11-24.
2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
7. Paladini, R.D. et al. (1996) J Cell Biol 132, 381-97.
8. Kim, S. et al. (2006) Nature 441, 362-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.