NLRC4 (D5Y8E) Rabbit mAb

• 产品编号：CST-12421S      品牌：CST       原厂货号：12421S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NLRC4 (D5Y8E) Rabbit mAb抗体能识别内源性NLRC4总蛋白水平。此单克隆抗体通过用合成肽免疫动物制备，该合成肽是人NLRC4蛋白Leu942周围的残基。核苷酸结合寡聚化结构域（NOD）样受体（NLR）蛋白家族是一种多样的细胞质先天性免疫受体家族，以一个氨基端效应者结构域为特征，常常是一个半胱天冬酶活化和募集结构域（CARD）或者一个pyrin结构域（PYD），后面有一个能识别病原体相关分子模式（PAMPs）的NOD和羧基端富含亮氨酸的重复单位（LRR）结构域（1）。NLR蛋白在先天性免疫应答过程中发挥多种作用，包括病原体传感、促炎细胞因子的转录激活（通过NF-κB）、I型干扰素的转录激活（通过IRFs）以及抗菌防卫体系的形成（引起炎性半胱氨酸天冬氨酸蛋白酶的激活）（1-7）。NLRC4（IPAF）抗菌防卫体系的形成适应细菌鞭毛蛋白和细菌保守的II型分泌系统(TTSS) 的成分(8-12)。配体检测和配体依赖的NLRC4低聚反应和防卫体系的激活需要NAIP蛋白质家族的参与（13,14）。在小鼠中，NAIP5和NAIP6与鞭毛蛋白相关，NAIP2与TTSS杆状蛋白相互作用（13,14）。在人上，NAIP识别TTSS针状蛋白Cprl（14）。另外，在细菌感染后NLRC4被PKCδ磷酸化，并且这种磷酸化作用是抗菌防卫体系的装配和caspase-1的活化所必需的（15）。

原厂资料：

Specificity / Sensitivity

NLRC4 (D5Y8E) Rabbit mAb recognizes endogenous levels of total NLRC4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu942 of human NLRC4 protein.

Background

The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family of proteins is a diverse family of cytoplasmic innate immune receptors. They are characterized by the presence of an amino-terminal effector domain, which is often either a caspase activation and recruitment domain (CARD) or a pyrin domain (PYD), followed by a NOD and carboxy-terminal leucine-rich-repeat (LRR) domain involved in recognition of pathogen-associated molecular patterns (PAMPs) (1). NLR proteins play a variety of roles during the innate immune response including pathogen sensing, transcriptional activation of proinflammatory cytokines through NF-κB, transcriptional activation of type I interferons through IRFs, and formation of inflammasomes leading to activation of inflammatory caspases (1-7).The NLRC4 (IPAF) inflammasome forms in response to bacterial flagellin as well as components of the bacterial conserved type II secretion system (TTSS) (8-12). Ligand detection and ligand-dependent NLRC4 oligomerization and inflammasome activation require the NAIP family of proteins (13,14). In mice, NAIP5 and NAIP6 associate with flagellin, while NAIP2 interacts with TTSS rod proteins (13,14). In humans, NAIP recognizes the TTSS needle protein Cprl (14). In addition, NLRC4 is phosphorylated by PKCδ in response to bacterial infection and this phosphorylation is required for inflammasome assembly and caspase-1 activation (15).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.