CK1δ Antibody

• 产品编号：CST-12417S      品牌：CST       原厂货号：12417S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CK1δ Antibody兔多抗能够检测内源性的CK1δ总蛋白。该抗体不会与CK1ε产生交叉反应。该多克隆抗体是由合成的人源CK1δ蛋白C末端肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。酪蛋白激酶I (CK1或CKI)是一个激酶家族的名字，这个家族蛋白由多个亚型组成(α, α', β, γ1-3, δ和ε)，带有一个保守的N末端激酶结构域和一个可变的C末端序列，该序列决定了蛋白的亚细胞定位并调控酶的活性（1-3）。事实上，已确定在CKIε 的C末端存在着多抑制自磷酸化位点（3）。这种广泛表达的蛋白激酶家族已被发现参与了包括DNA修复，细胞形态学，Wnt信号在内的多个生物学过程（4）。可能被研究的最明确的CKI的作为是起始β-catenin45位丝氨酸磷酸化，产生GSK3底物基序(丝氨酸/苏氨酸-X-X-X-磷酸化丝氨酸)。CK1δ参与许多细胞过程，如分化（5），细胞生长和细胞凋亡（6-8）以及昼夜节律的控制（9）。

1. Gross, S.D. and Anderson, R.A. (1998) Cell. Signal. 10, 699-711.
2. Vancura, A. et al. (1994) J. Biol. Chem. 269, 19271-19278.
3. Gietzen, K.F. and Virshup, D.M. (1999) J. Biol. Chem. 274, 32063-32070.
4. Polakis, P. (2002) Curr. Biol. 12, R499-R501.
5. Bryja, V. et al. (2007) J Cell Sci 120, 586-95.
6. Knippschild, U. et al. (2005) Onkologie 28, 508-14.
7. Knippschild, U. et al. (1997) Oncogene 15, 1727-36.
8. Brockschmidt, C. et al. (2008) Gut 57, 799-806.
9. Vanselow, K. and Kramer, A. (2007) Cold Spring Harb Symp Quant Biol 72, 167-76.

原厂资料：

Specificity / Sensitivity

CK1δ Antibody recognizes endogenous levels of total CK1δ protein. This antibody does not cross-react with CK1ε.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CK1δ protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Casein Kinase I (CK1 or CKI) is the name given to a family of kinases consisting of multiple isoforms (α, α', β, γ1-3, δ, and ε) with a conserved N-terminal kinase domain and a variable C-terminal sequence that determines subcellular localization and regulates enzyme activity (1-3). Indeed, multiple inhibitory autophosphorylation sites have been identified near the C terminus of CK1ε (3). This ubiquitously expressed family of protein kinases has been implicated in multiple processes including DNA repair, cell morphology, and Wnt signaling (4). Perhaps the best understood role of CK1 is to provide the priming phosphorylation of β-catenin at Ser45 to produce the consensus GSK-3 substrate motif (S/T-X-X-X-pS) (4).CK1δ is involved in many cellular processes such as differentiation (5), cell growth and apoptosis (6-8), and control of the circadian rhythm (9).

1. Gross, S.D. and Anderson, R.A. (1998) Cell. Signal. 10, 699-711.
2. Vancura, A. et al. (1994) J. Biol. Chem. 269, 19271-19278.
3. Gietzen, K.F. and Virshup, D.M. (1999) J. Biol. Chem. 274, 32063-32070.
4. Polakis, P. (2002) Curr. Biol. 12, R499-R501.
5. Bryja, V. et al. (2007) J Cell Sci 120, 586-95.
6. Knippschild, U. et al. (2005) Onkologie 28, 508-14.
7. Knippschild, U. et al. (1997) Oncogene 15, 1727-36.
8. Brockschmidt, C. et al. (2008) Gut 57, 799-806.
9. Vanselow, K. and Kramer, A. (2007) Cold Spring Harb Symp Quant Biol 72, 167-76.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody