Malic Enzyme (Mitochondrial) Antibody

• 产品编号：CST-12399S      品牌：CST       原厂货号：12399S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Malic Enzyme (Mitochondrial) Antibody 能够检测内源性苹果酸酶（线粒体）总蛋白水平。该多克隆抗体是由合成的人源苹果酸酶（线粒体）蛋白甘氨酸(450位)肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。胞浆苹果酸酶（ME1）催化苹果酸和NADP+转化为丙酮酸和NADPH（1,2）。然后NADPH被用于脂肪酸的生物合成和脂肪生成（1,2）。已证实胞浆苹果酸酶介导了高脂饮食诱导的肥胖（1）。线粒体苹果酸酶（ME2）优先利用NAD+催化苹果酸转化成丙酮酸，同时伴随着新的NADH的产生（2）。最近的研究表明，肿瘤抑制基因p53通过抑制胞浆苹果酸酶和线粒体苹果酸酶的表达来调节细胞代谢和增殖（3）。

1. Al-Dwairi, A. et al. (2012) PLoS One 7, e46716.
2. Pongratz, R.L. et al. (2007) J Biol Chem 282, 200-7.
3. Jiang, P. et al. (2013) Nature 493, 689-93.

原厂资料：

Specificity / Sensitivity

Malic Enzyme (Mitochondrial) Antibody recognizes endogenous levels of total malic enzyme (mitochondrial) protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly450 of human malic enzyme (mitochondrial) protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cytosolic malic enzyme (ME1) catalyzes the conversion of malate and NADP+ to pyruvate and NADPH (1,2). NADPH is then used for fatty acid biosynthesis and lipogenesis (1,2). Cytosolic malic enzyme was shown to mediate high fat diet-induced adiposity (1). Mitochondrial malic enzyme (ME2) preferentially uses NAD+ to catalyze the conversion of malate to pyruvate with the concomitant generation of NADH (2). Recent studies have demonstrated that the tumor suppressor p53 regulates cell metabolism and proliferation by repressing the expression of both cytosolic malic enzyme and mitochondrial malic enzyme (3).

1. Al-Dwairi, A. et al. (2012) PLoS One 7, e46716.
2. Pongratz, R.L. et al. (2007) J Biol Chem 282, 200-7.
3. Jiang, P. et al. (2013) Nature 493, 689-93.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.