Phospho-DNAJC2/MPP11 (Ser47) Antibody

• 产品编号：CST-12397S      品牌：CST       原厂货号：12397S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-DNAJC2/MPP11 (Ser47) Antibody能够检测内源性的仅在 Ser47位点磷酸化的 DNAJC2/MPP11 蛋白。该多克隆抗体是经由合成的围绕人和小鼠DNAJC2/MPP11 蛋白 Ser47位点的氨基酸肽段免疫动物而生产的。经过Protein A和亲和层析纯化。DnaJ/Hsp40蛋白是一个含有J结构域的分子伴侣蛋白的保守家族，其可通过他们与Hsp70分子伴侣蛋白的相互作用来协助蛋白折叠和稳定（综述1）。DNAJC2,也称为MPP11 (M-phase phosphoprotein 11 protein)是核糖体相关复合物(RAC)的一个组分。RAC定位在胞质内，在这里他可以通过刺激Hsp70分子伴侣蛋白的ATPase活性来协助维持新合成多肽的正确折叠（2,3）。在核内，MPP11通过介导从polycomb抑制状态到活化的染色质的开关来参与转录激活（4）。之前的研究表明MPP11在白血病，头部及颈部癌症中过表达，致使研究者认为MPP11可能是一个潜在的治疗靶点（5-7）。Phospho-DNAJC2/MPP11 (Ser47) Antibody能够指向一个位点，其是Cell Signaling Technology (CST)使用PhosphoScan®平台发现的。这个平台是基于LC-MS/MS的修饰位点发现平台。更多信息请访问PhosphoSitePlus®, CST's修饰位点数据知识库, www.phosphosite.org。

1. Qiu, X.B. et al. (2006) Cell Mol Life Sci 63, 2560-70.
2. Hundley, H.A. et al. (2005) Science 308, 1032-4.
3. Otto, H. et al. (2005) Proc Natl Acad Sci U S A 102, 10064-9.
4. Richly, H. et al. (2010) Nature 468, 1124-8.
5. Greiner, J. et al. (2003) Int J Cancer 106, 224-31.
6. Resto, V.A. et al. (2000) Cancer Res 60, 5529-35.
7. Tabarkiewicz, J. and Giannopoulos, K. (2010) Transplant Proc 42, 3293-6.

原厂资料：

Specificity / Sensitivity

Phospho-DNAJC2/MPP11 (Ser47) Antibody recognizes endogenous levels of DNAJC2/MPP11 protein only when phosphorylated at Ser47.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser47 of human and mouse DNAJC2/MPP11 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

DnaJ/Hsp40 proteins are a conserved family of J-domain-containing chaperone proteins that assist in protein folding and stability through their interactions with Hsp70 chaperone proteins (reviewed in 1). DNAJC2, also known as MPP11 (M-phase phosphoprotein 11 protein), is a component of the ribosome-associated complex (RAC). The RAC is localized to the cytoplasm, where it assists in maintaining appropriate folding of nascent polypeptides by stimulating the ATPase activity of Hsp70 chaperone proteins (2,3). In the nucleus, MPP11 is involved in the activation of transcription through mediation of the switch from polycomb-repressed to active chromatin (4). Previous studies have shown MPP11 is overexpressed in leukemia and head and neck cancer, leading researchers to suggest MPP11 may be a potential therapeutic target (5-7). 
 
 Phospho-DNAJC2/MPP11 (Ser47) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery. Please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org for more information.

1. Qiu, X.B. et al. (2006) Cell Mol Life Sci 63, 2560-70.
2. Hundley, H.A. et al. (2005) Science 308, 1032-4.
3. Otto, H. et al. (2005) Proc Natl Acad Sci U S A 102, 10064-9.
4. Richly, H. et al. (2010) Nature 468, 1124-8.
5. Greiner, J. et al. (2003) Int J Cancer 106, 224-31.
6. Resto, V.A. et al. (2000) Cancer Res 60, 5529-35.
7. Tabarkiewicz, J. and Giannopoulos, K. (2010) Transplant Proc 42, 3293-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.