hnRNP C1/C2 Antibody

• 产品编号：CST-12392S      品牌：Cell Signaling Technology       原厂货号：12392S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

hnRNP C1/C2 能够检测内源性的hnRNP C1/C2总蛋白水平。该抗体不会与其他hnRNPCL1或 RALY蛋白发生交叉反应。该多克隆抗体是经由合成的围绕人 hnRNP C1/C2 蛋白近氨基端的肽段免疫动物而生产的。经过Protein A和亲和层析纯化。Heterogeneous nuclear ribonucleoprotein C1/C2 (hnRNP C1/C2)具有多种生物学功能如转录调控、DNA修复以及RNA加工路径。hnRNP C1/C2可作为mRNA加工路径中的“分子标尺”，其负责一旦新合成的转录本长度超过200-300个核苷酸，就会被从染色质模板上提交至mRNA输出路径中（1）。研究显示若没有hnRNP C1/C2， LCR相关的重塑复合物 (LARC)就不能与其靶DNA序列联系在一起（2,3）。LARC可以调控红细胞生成过程中β-globin基因的转录（2,3）。hnRNP C1/C2与其他hnRNP蛋白一样，能够与DNA损伤反应(DDR)蛋白相互作用（4）。hnRNP蛋白可以通过促进同源重组(HR)或非同源末端连接(NHEJ)从而调控双链断裂(DSB)修复（4）。hnRNP C1/C2可下调miR-21的表达，miR-21的表达能够导致多形性成胶质细胞瘤 (GBM)中程序性细胞死亡4 (PDCD4)的升高表达（5）。研究还发现hnRNP C1/C2沉默后可致使GBM细胞更易发生凋亡（5）。

1. McCloskey, A. et al. (2012) Science 335, 1643-6.
2. Huang, L. et al. (2011) Mol Cell Biol 31, 3472-84.
3. Mahajan, M.C. et al. (2005) Proc Natl Acad Sci U S A 102, 15012-7.
4. Haley, B. et al. (2009) Int J Radiat Biol 85, 643-55.
5. Park, Y.M. et al. (2012) Mol Cell Biol 32, 4237-44.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Xenopus, Bovine, Dog, Pig, Guinea Pig, Horse

Specificity / Sensitivity

hnRNP C1/C2 Antibody recognizes endogenous levels of total hnRNP C1/C2 protein. This antibody does not cross-react with other hnRNPCL1 or RALY proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human hnRNP C1/C2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Heterogeneous nuclear ribonucleoprotein C1/C2 (hnRNP C1/C2) has multiple biological functions including transcriptional regulation, DNA repair, and RNA processing pathways. hnRNP C1/C2 acts as a ‘molecular ruler’ in the mRNA processing pathway, committing nascent transcripts from the chromatin template to the mRNA export pathway once the nascent transcript becomes longer than 200-300 nucleotides (1). Studies indicate that without hnRNP C1/C2, the LCR-associated remodeling complex (LARC) does not associate with its target DNA sequence (2,3). LARC regulates the transcription of β-globin genes during erythropoiesis (2,3). hnRNP C1/C2, as well as other hnRNP proteins, interact with DNA damage response (DDR) proteins (4). hnRNP proteins regulate double stranded break (DSB) repair by promoting either homologous recombination (HR) or non-homologous end joining (NHEJ) (4). hnRNP C1/C2 down-regulates the expression of miR-21, which leads to the increased expression of programmed cell death 4 (PDCD4) protein in glioblastoma multiforme (GBM) (5). Research studies have shown that silencing of hnRNP C1/C2 renders GBM cells more susceptible to apoptosis (5).

1. McCloskey, A. et al. (2012) Science 335, 1643-6.
2. Huang, L. et al. (2011) Mol Cell Biol 31, 3472-84.
3. Mahajan, M.C. et al. (2005) Proc Natl Acad Sci U S A 102, 15012-7.
4. Haley, B. et al. (2009) Int J Radiat Biol 85, 643-55.
5. Park, Y.M. et al. (2012) Mol Cell Biol 32, 4237-44.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.