GFAP (D1F4Q) XP? Rabbit mAb

• 产品编号：CST-12389S      品牌：CST       原厂货号：12389S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GFAP (D1F4Q) XP® Rabbit mAb兔单抗能够检测内源性的GFAP总蛋白。该单克隆抗体是由合成的人源GFAP蛋白天冬氨酸(395位)肽段免疫动物而制备的。细胞骨架由三种胞内纤维构成：微丝（肌动蛋白纤维），中间纤维和微管。中间纤维的主要类型是在特定类型的细胞中特异性表达的：包括上皮细胞的细胞角蛋白，神经胶质细胞的胶质纤维酸性蛋白（GFAP），骨骼、内脏和某些血管平滑肌细胞中的结蛋白，间充质来源细胞中的波形蛋白和神经元中的神经丝。GFAP和波形蛋白在星形胶质细胞中形成中间纤维，调节它们的运动和形状（1）。波形蛋白纤维多存在于早期发育阶段，而GFAP纤维是脑星形胶质细胞分化和成熟的特征。因此，GFAP通常用来作为星形胶质细胞所引起的颅内及椎管内肿瘤的标记（2）。此外，GFAP中间纤维也在周围神经系统非髓鞘形成的雪旺氏细胞中存在（3）。

1. Eng, L.F. et al. (2000) Neurochem. Res. 25, 1439-51.
2. Goebel, H.H. et al. (1987) Acta. Histochem. Suppl. 34, 81-93.
3. Jessen, K.R. et al. (1990) Development 109, 91-103.

原厂资料：

Specificity / Sensitivity

GFAP (D1F4Q) XP® Rabbit mAb recognizes endogenous levels of total GFAP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp395 of human GFAP protein.

Background

The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral, and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin, and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system (3).

1. Eng, L.F. et al. (2000) Neurochem. Res. 25, 1439-51.
2. Goebel, H.H. et al. (1987) Acta. Histochem. Suppl. 34, 81-93.
3. Jessen, K.R. et al. (1990) Development 109, 91-103.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.