Thyroid Transcription Factor 1 (TTF-1) (D2E8) Rabbit mAb

• 产品编号：CST-12373S      品牌：CST       原厂货号：12373S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

甲状腺转录因子1（D2E8）Rabbit mAb兔单抗可以识别内源性的总甲状腺转录因子1蛋白。甲状腺转录因子1（D2E8）Rabbit mAb兔单抗可以识别内源性的总甲状腺转录因子1蛋白。此单克隆抗体由合成肽段免疫动物产生，该肽段与人甲状腺转录因子1蛋白的邻近氨基末端残基序列一致。甲状腺转录因子1（TTF1，也称为NKX2-1），是NKX同源转录因子家族的一员，最初在FRTL-5大鼠甲状腺细胞系中发现（1）。随后的研究显示TTF1在发育的甲状腺，肺和前脑腹侧的分化和形态建成过程中都有重要作用（2）。TTF1控制了多个基因的表达，部分是组织特异性的，例如：甲状腺球蛋白，甲状腺过氧化物酶，和促甲状腺素受体；以及肺的表面活性蛋白和克拉氏细胞上的分泌蛋白（2,3）。研究发现TTF1在甲状腺和肺的恶性肿瘤中会表达，通常被用于早期和恶性肺癌的标记（4-6）。

1. Civitareale, D. et al. (1989) EMBO J 8, 2537-42.
2. Boggaram, V. (2009) Clin Sci (Lond) 116, 27-35.
3. Yamaguchi, T. et al. (2012) Cancer Cell 21, 348-61.
4. Whithaus, K. et al. (2012) Arch Pathol Lab Med 136, 155-62.
5. Yoshida, A. et al. (2011) Lung Cancer 72, 309-15.
6. Moldvay, J. et al. (2004) Pathol Oncol Res 10, 85-8.

原厂资料：

omology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Thyroid Transcription Factor 1 (D2E8) Rabbit mAb recognizes endogenous levels of total thyroid transcription factor 1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human thyroid transcription factor 1 protein.

Background

Thyroid Transcription Factor 1 (TTF1, also known as NKX2-1), a member of the NKX homeobox transcription factor family, was initially discovered in the FRTL-5 rat thyroid cell line (1). Subsequent studies have shown that TTF1 plays an important role in differentiation and morphogensis of the developing thyroid, lung, and ventral forebrain (2). TTF1 controls the expression of several genes, some of which are tissue specific, such as: thyroglobulin, thyroperoxidase, and the thyrotropin receptor in the thyroid; and surfactant proteins and clara cell secretory protein in the lung (2,3). Investigators have found that TTF1 is expressed in malignant tumors of the thyroid and lung, and it is commonly used as a marker for both primary and malignant lung cancers (4-6).

1. Civitareale, D. et al. (1989) EMBO J 8, 2537-42.
2. Boggaram, V. (2009) Clin Sci (Lond) 116, 27-35.
3. Yamaguchi, T. et al. (2012) Cancer Cell 21, 348-61.
4. Whithaus, K. et al. (2012) Arch Pathol Lab Med 136, 155-62.
5. Yoshida, A. et al. (2011) Lung Cancer 72, 309-15.
6. Moldvay, J. et al. (2004) Pathol Oncol Res 10, 85-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.