Nitrocellulose Sandwiches

• 产品编号：CST-12369S      品牌：CST       原厂货号：12369S
• 产品分类：蛋白质检测与分析 > 蛋白质印迹分析 > 蛋白印迹膜和滤纸
• 应用分类：

描述：

硝酸纤维素膜尺寸： 7× 8.5cm，孔径：0.2μm，结合能力： 90 μg/cm2CST公司的Nitrocellulose Sandwiches是预包装的sandwiches，每包包含一张预切好的硝酸纤维素膜和2张0.34mm厚的印迹纸。为了提供便利和节省时间，已经用CST的western blot方案进行了验证，并且CST的科研工作者也首选此产品。Nitrocellulose Sandwiches是结合不同分子量的蛋白的理想产品。0.2 μm的孔径使低分子量的蛋白也能被高度保留下来，甚至是低于20kDa的蛋白。Nitrocellulose Sandwiches对于大多数常用的转印方法都适用，包括槽、半干和真空印迹技术。本产品还适用于色度法、荧光和化学发光检测。对于化学发光检测，CST推荐基于HRP的系统。与PVDF膜不一样，硝酸纤维素不需要甲醇预先浸湿这个步骤。

原厂资料：

Description

Nitrocellulose Membrane Dimensions: 7 X 8.5 cm, Pore Size: 0.2 μm, Binding Capacity: 90 μg/cm2

Background

Nitrocellulose Sandwiches from Cell Signaling Technology® are pre-packaged sandwiches (20/pack) consisting of a precut nitrocellulose membrane and 2 sheets of 0.34 mm thick blotting paper. Intended to provide convenience and time savings, they are also validated for use in CST's Western Blot protocol and are the preferred membrane used by Cell Signaling Technology scientists. Nitrocellulose Sandwiches are ideal for binding proteins of all molecular weights; the 0.2 μm pore size ensures high retention of low molecular weight proteins, even below 20 kDa. Nitrocellulose Sandwiches are suitable for most common transfer methods including tank, semi-dry, and vacuum blotting. This product is also suitable for colorimetric, fluorescent, and chemiluminescent detection. For chemiluminescent detection, CST recommends HRP based systems. Unlike PVDF, nitrocellulose does not require a methanol pre-wetting step.

注意事项：

Storage: Store at room temperature in a cool and dry place
away from light or heat sources. Nitrocellulose is extremely
flammable and must be kept away from fire to avoid ignition.
Stored appropriately, the product is stable for 24 months.
Handle with gloves; nitrocellulose is highly absorbent and will
bind proteins from skin contact which may reduce performance
and decrease the quality of data.