DLAT (4A4-B6-C10) Mouse mAb

• 产品编号：CST-12362S      品牌：CST       原厂货号：12362S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DLAT (4A4-B6-C10) Mouse mAb 能够检测内源性DLAT总蛋白水平。该单克隆抗体是由重组人源DLAT蛋白亮疫动物而制备的。二氢硫辛酰胺乙酰转移酶（DLAT）催化一个乙酰基从丙酮酸转移到辅酶A，进而合成乙酰辅酶A（1-4）。已知这种蛋白质是丙酮酸脱氢酶复合物E2亚单位（PDC-E2），文献提到其在原发性胆汁性肝硬化中是主要的自身抗原（2-5）。当DLAT暴露在胆管凋亡细胞的免疫系统中时，有可能形成抗线粒体抗体（AMAS）（3,5）。研究表明，在某些情况下，化妆品，非甾体抗炎药，口香糖，对乙酰氨基酚和其它化合物可能会引发敏感个体DLAT的暴露（3）。 AMAS的存在疾病诊断前往往被检测到（4,5）。研究还表明，激活的Toll样受体（TLR-3）通路参与从亚临床状态到临床状态的变化（4）。

1. Thekkumkara, T.J. et al. (1988) FEBS Lett 240, 45-8.
2. Coppel, R.L. et al. (1988) Proc Natl Acad Sci U S A 85, 7317-21.
3. Naiyanetr, P. et al. (2011) J Autoimmun 37, 209-16.
4. Ambrosini, Y.M. et al. (2011) Clin Exp Immunol 166, 110-20.
5. Lleo, A. et al. (2009) Hepatology 49, 871-9.

原厂资料：

Specificity / Sensitivity

DLAT (4A4-B6-C10) Mouse mAb recognizes endogenous levels of total DLAT protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human DLAT protein.

Background

Dihydrolipoamide acetyltransferase (DLAT) transfers an acetyl group from pyruvate to CoA to synthesize acetyl-CoA (1-4). This protein, also known as the E2 subunit of the pyruvate dehydrogenase complex (PDC-E2), has been implicated in the literature as the primary autoantigen in primary biliary cirrhosis (2-5). Antimitochondrial antibodies (AMAs) are likely formed when DLAT is exposed to the immune system in apoptotic cells of the bile duct (3,5). Research studies have shown that in some cases, cosmetics, NSAIDs, chewing gum, acetaminophen, and other compounds could trigger exposure of DLAT in sensitive individuals (3). The presence of AMAs is often detectable before disease diagnosis (4,5). Research studies have also shown that activation of the Toll-like receptor-3 (TLR-3) pathway is involved in the progression from a subclinical to clinical state (4).

1. Thekkumkara, T.J. et al. (1988) FEBS Lett 240, 45-8.
2. Coppel, R.L. et al. (1988) Proc Natl Acad Sci U S A 85, 7317-21.
3. Naiyanetr, P. et al. (2011) J Autoimmun 37, 209-16.
4. Ambrosini, Y.M. et al. (2011) Clin Exp Immunol 166, 110-20.
5. Lleo, A. et al. (2009) Hepatology 49, 871-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.