SAMHD1 Antibody

• 产品编号：CST-12361S      品牌：CST       原厂货号：12361S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SAMHD1抗体能识别内源性SAMHD1总蛋白水平，此抗体与来源不明的30kDa的蛋白有交叉反应。此多克隆抗体通过用合成肽免疫动物制备，该合成肽是人SAMHD1蛋白Pro607周围的残基。抗体通过蛋白A以及多肽亲和色谱法纯化。SAM结构域和HD结构域蛋白1（SAMHD1）是细胞内在的先天性免疫应答的“负调节器”（1）。研究表明SAMHD1突变能引起Aicardi-Goutieres综合征，这是一种自身免疫病，以干扰素α增加为特征，症状类似于先天性病毒感染（1）。最近，SAMHD1被认为是一种限制因子，使许多髓系细胞能够抵抗人免疫缺陷病毒（HIV）的感染（2-4）。抗感染细胞中病毒蛋白Vpx的表达能使SAMHD1进行由泛素介导的降解，解除对HIV的限制。SAMHD1通过水解细胞内的三磷酸脱氧核糖核苷(dNTPs)来防止自身免疫和HIV感染，限制自身核酸引起的不适当的免疫激活以及抑制HIV基因组的反转录（4-6）。

原厂资料：

Specificity / Sensitivity

SAMHD1 Antibody recognizes endogenous levels of total SAMHD1 protein. This antibody cross-reacts with a 30 kDa protein of unknown origin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro607 of human SAMHD1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

SAM domain and HD domain-containing protein 1 (SAMHD1) is a negative regulator of the cell-intrinsic innate immune response (1). Research studies have identified mutations in SAMHD1 as a cause of Aicardi-Goutieres syndrome, an autoimmune disease characterized by elevated production of interferon-α and symptoms resembling congenital viral infection (1). Recently, SAMHD1 was identified as the restriction factor that renders most myeloid cells refractory to infection by human immunodeficiency virus (HIV) (2-4). Expression of the viral protein Vpx in refractory cells targets SAMHD1 for ubiquitin-mediated degradation and relieves HIV restriction. SAMHD1 prevents autoimmunity and HIV infection by hydrolizing intracellular deoxynucleoside triphosphates (dNTPs), thereby limiting inappropriate immune activation by self nucleic acid and inhibiting reverse transcription of the HIV genome (4-6).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.