ARID1A/BAF250A (D2A8U) Rabbit mAb

• 产品编号：CST-12354S      品牌：CST       原厂货号：12354S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ARID1A/BAF250A(D2A8U) Rabbit mAb兔单抗能够检测内源性的ARID1A/BAF250A总蛋白水平。该抗体还能与未知来源的分子量为65kD的蛋白发生交叉反应。该单克隆抗体是采用合成的与人源ARDI1A/BAF250A蛋白Gly293残基周围序列相对应的肽段免疫动物而生产的。ATP依赖的染色质重塑复合物，在调节如转录、DNA的复制和修复等细胞核反应起着重要作用(1,2)。SWI/SNF染色质重塑复合物由10多个亚基组成，包含BRM或BRG1任一单分子作为ATP酶催化亚基。ATP酶亚基活性能够破坏组蛋白与DNA的结合，同时改变染色质重要调控元件与染色质的结合。额外的核心和配体亚基起着支架作用，有助于稳定各种转录因子和染色质的相互作用，为其提供作用表面(2-5)。SWI/SNF亚基与各种转录因子如核受体，p53, Rb, BRCA1和MyoD等的相互作用，有利于招募复合物结合目标基因，对基因的激活、细胞生长、细胞周期和分化过程进行调控(1,6-9)。ARID1A是SWI/SNF复合物的亚基。SWI/SNF复合物是esBAF中必不可缺的一部分(小鼠胚胎干细胞特异的SWI/SNF复合物)。ARID1A对于ES细胞的多能性以及分化称为中胚层衍生的心肌细胞和脂肪细胞起着关键作用(10)。另外，ARID1A在多种肿瘤如卵巢癌、膀胱癌和胃癌等存在频繁基因突变(11-13)。

1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
9. Simone, C. (2006) J Cell Physiol 207, 309-14.
10. Gao, X. et al. (2008) Proc Natl Acad Sci U S A 105, 6656-61.
11. Jones, S. et al. (2010) Science 330, 228-31.
12. Wiegand, K.C. et al. (2010) N Engl J Med 363, 1532-43.
13. Guan, B. et al. (2011) Am J Surg Pathol 35, 625-32.

原厂资料：

Specificity / Sensitivity

ARID1A/BAF250A (D2A8U) Rabbit mAb recognizes endogenous levels of total ARID1A/BAF250A protein. This antibody also cross-reacts with proteins of unknown origin at 65 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1293 of human ARID1A/BAF250A protein.

Background

ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9).ARID1A is one of the accessory subunits of the SWI/SNF complex that is an essential part of the esBAF (mouse embryonic stem cell specific SWI/SNF complex). ARID1A is critical for ES cell pluripotency and differentiation into mesoderm-derived cardiomyocytes and adipocytes (10). In addition, ARID1A has been found to be frequently mutated in several cancers such as uterine and ovarian endometrioid carcinoma and ovarian clear cell carcinoma (11-13).

1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
9. Simone, C. (2006) J Cell Physiol 207, 309-14.
10. Gao, X. et al. (2008) Proc Natl Acad Sci U S A 105, 6656-61.
11. Jones, S. et al. (2010) Science 330, 228-31.
12. Wiegand, K.C. et al. (2010) N Engl J Med 363, 1532-43.
13. Guan, B. et al. (2011) Am J Surg Pathol 35, 625-32.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.