ERCC1 (D6G6) XP? Rabbit mAb

• 产品编号：CST-12345S      品牌：CST       原厂货号：12345S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ERCC1 splice variants around 30 kDa.ERCC1 (D6G6) XP®Rabbit mAb可以识别内源性总的ERCC1蛋白。抗体可以检测30kDa左右的基因剪接突变体。单克隆抗体由合成肽段免疫动物产生，该肽段与人ERCC1蛋白的羧基端残基序列一致。所有活细胞都有DNA损伤修复功能以调控DNA损伤。核苷酸切除修复出现在大片段的螺旋-扭曲损伤时，例如由UV或某种化学物质引发的损伤（1）。Excision Repair Cross Complementing 1 (ERCC1)与XPF构成复合物，作为5’内切酶切除掉发生损伤的部位（2）。ERCC1-XPF还在DNA链间交联（ICLs）（3）和双链损伤时（4）发挥足以。研究证明ERCC1的表达与存活率相关，也在一些人类癌症疾病，包括肺小细胞癌（NSCLC）对化疗的的反应过程相关（5,6）。

1. Shuck, S.C. et al. (2008) Cell Res 18, 64-72.
2. McDaniel, L.D. and Schultz, R.A. (2008) Adv Exp Med Biol 637, 65-82.
3. Niedernhofer, L.J. et al. (2004) Mol Cell Biol 24, 5776-87.
4. Ahmad, A. et al. (2008) Mol Cell Biol 28, 5082-92.
5. Zheng, Z. et al. (2007) N Engl J Med 356, 800-8.
6. Gossage, L. and Madhusudan, S. (2007) Cancer Treat Rev 33, 565-77.

原厂资料：

Specificity / Sensitivity

ERCC1 (D6G6) XP® Rabbit mAb recognizes endogenous levels of total ERCC1 protein. This antibody also detects ERCC1 splice variants around 30 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ERCC1 protein.

Background

DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions occur, such as those brought about by UV and certain chemicals (1). Excision Repair Cross Complementing 1 (ERCC1) forms a complex with XPF, which acts as the 5’ endonuclease required to excise the lesion (2). ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) (3) and involved in repair of double strand breaks (4). Research studies have shown that expression of ERCC1 is related to survival rate and response to chemotherapeutic drugs in several human cancers including non-small cell lung cancer (NSCLC) (5,6).

1. Shuck, S.C. et al. (2008) Cell Res 18, 64-72.
2. McDaniel, L.D. and Schultz, R.A. (2008) Adv Exp Med Biol 637, 65-82.
3. Niedernhofer, L.J. et al. (2004) Mol Cell Biol 24, 5776-87.
4. Ahmad, A. et al. (2008) Mol Cell Biol 28, 5082-92.
5. Zheng, Z. et al. (2007) N Engl J Med 356, 800-8.
6. Gossage, L. and Madhusudan, S. (2007) Cancer Treat Rev 33, 565-77.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.